摘要
为了检测人疱疹病毒６型（ＨＨＶ－６）在器官移植中的致病性，作者对１２例肾脏移植受者在经基础免疫治疗３周后，取外周血淋巴细胞与脐血淋巴细胞共同培养。结果显示，２例受者标本中见有细胞病变（ＣＰＥ），其中１株病毒经多次传代冻存后仍能产生ＣＰＥ。该病毒感染细胞超薄切片电镜下见疱疹病毒样颗粒，感染细胞涂片与抗ＨＨＶ－６单克隆抗体产生明显荧光，证实为ＨＨＶ－６。作者认为，ＨＨＶ－６可能在肾脏移植后感染，尤其在排异反应中起重要作用。
One strain of the viruses was isolated from preipheral blood lymphocytes(PBL) of a renal transplant recipient.PBL isolated from blood samples were cocultured with the PHA actived cord blood lymphocytes(CBL).Two of twelve recipient's samples found cytopathic effect after 10 to 14 days. Examination of ultrathin sections of the virus infected cells by electron microscope showed herpes like virus particles.Detection of indirect immunofluorescences with McAbs against HHV 6 was positive in the infected cells.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1997年第8期499-500,I071,共3页
Chinese Journal of Surgery
