摘要
目的研究生长激素（GH）基因多态性与青少年特发性脊柱侧凸（AIS）发生发展的关系。方法本研究包括265例AIS患者及193名正常对照。在AIS患者组，记录其最大Cobb角。采用PCR-RFLP的方法对GH基因启动子区域多态性位点rs2854184进行基因分型。结果在AIS患者组，GH基因rs2854184多态性位点的3个基因型AA、AT、TT分别占38．3％，50．3％，11．4％，正常对照组分别占39．6％，50．2％，10．1％，2组比较差异无统计学意义。同样，在AIS患者组rs2854184多态性位点的2个等位基因A、T分别占63．5％，36．5％，正常对照组分别占64．7％，35．3％，2组比较差异无统计学意义。另外在AIS组内，多态性位点rs2854184不同基因型所对应最大Cobb角分别是AA33．8°±10．0°，AT36．4°±15．0°，TT34．5°±9．1°，3者比较差异无统计学意义。结论GH基因rs2854184位点多态性与AIS的发生发展没有明显关系。
Objective To investigate whether the growth hormone gene （GH） promotor polymorphism （rs2854184） is associated with the occurrence or curve severity of adolescent idiopathic scoliosis （AIS）. Methods Two hundred and sixty-five AIS patients and 193 normal controls were recruited. The maximum Cobb angles were recorded in AIS patients. PCR-RFLP was used for the genotyping. Results The genotype frequency distribution were AA 38.3%, AT 50. 3%, TT 11.4% in AIS patients and AA 39. 6%, AT 50. 2%, 10. 1% TT in controls for the promotor polymorphism rs2854184 in GH gene. It was comparable between AIS and normal control. The allele frequency distribution was also comparable between AIS and normal control. It was 63.5% for allele A, 36. 5% for allele T in AIS patients and 64.7% for allele A, 35.3% for allele T in normal control. The mean maximum Cobb angle in AIS patients with AA, AT, TT genotypes were 33.8°±10. 0°, 36.4°±15.0°, 34. 5°±9. 1°, respectively, it was similar with each other. Conclusion The GH gene promoter polymorphism is neither associated with the occurrence nor the curve severity of AIS.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2008年第22期1741-1743,共3页
Chinese Journal of Surgery
基金
基金项目：江苏省国际科技合作项目（BZ2007024）
香港研资局基金（RGC grants）（CUHK4337/04M）
