摘要
研究尿激酶(uPA)、尿激酶受体(uPAR)在人脑胶质瘤中的表达特征,评估其在胶质瘤恶性生长方式中的作用。选取57例不同恶性程度的星形细胞瘤、13例良性脑膜瘤和10例正常脑组织,分别以抗uPA单克隆抗体和抗uPAR多克隆抗体用ABC法进行免疫组织化学染色和半定量分析。结果表明:uPA、uPAR的表达程度与胶质瘤的恶性程度有关,胶质瘤恶性程度越高,表达程度越高。良性脑膜瘤的表达程度较低。正常脑组织则未见uPA、uPAR的表达。uPA染色除见于肿瘤细胞、血管内皮细胞外,还见于少数胶质增生带中的正常胶质细胞和神经元。在肿瘤细胞增殖密集区域,uPA阳性的肿瘤细胞比例较低,而在肿瘤细胞生长较稀疏、肿瘤血管增殖旺盛的区域阳性细胞比例较高。uPAR的染色定位于肿瘤细胞和血管内皮细胞。uPA和uPAR的共同表达是浸润性生长的胶质瘤的特征之一。其表达程度可作为判断星形细胞瘤浸润性的重要参考指标。在人体内,表达uPA的细胞种类是较多的。uPA、uPAR的表达对胶质瘤的浸润和血管发生起着重要作用。
To study the patterns of the expression of urokinase (uPA), urokinase receptor (uPAR) in human gliomas and their roles in malignant phenotype of gliomas. The immuno-histochemical localization of uPA and uPAR was evaluated by using the avidin-biotin im-munoperoxidase technique. Studies were performed in 70 surgically excised specimens, including57 astrocytomas, 13 meningiomas and 10 normal brain tissue. The immunohis-tochemical staining results were densitometrically semiquantitatived. The intensity of the im-munoreaction correlated with biological malignancy of astrocytoma. A semiquantitative analysisof immunohistochemical results showed that uPA and uPAR were overexpressed in Ⅲ -Ⅳ grade astrocytomas in comparison with Ⅰ-Ⅱ grade astocytomas or meningiomas, and they were undetectable in normal brain tissue. The staining for uPA was mainly localized on tumor cells and vascular endothelial cells, but was also seen on a few normal glial cells and neurons adjacent to glioma tissue. The uPA staining was hetrogeneously distributed with preferential localization in the areas of vascular proliferation. The astrocytoma cells in denser proliferated areas showed minor uPA positivity. The staining for uPAR was localized on as- trocytoma cells and endothelial cells. Overexpression of uPA and uPAR is a constant feature of invasive malignant astrocytomas in vivo. The cellular source of uPA was not only from as-trocytoma cells and endothelial cells, but also from some other cells, maybe including normal glial cells and neurons. The combined increased expression of uPA and its cellular receptor, uPAR, may account for the activasion of the proteolytic system which occurs in invasion and angiogenesis of astrocytoma.
出处
《临床神经科学》
1997年第4期191-195,共5页
Chinese Journal of Clinical Neurosciences
