摘要
目的研究完全性睡眠剥夺对幼鼠学习记忆的影响，并对其可能的神经学机制进行深入探讨。方法将12只出生后3周刚断奶的雄性SD幼鼠随机分为实验组和对照组。利用小站台水平面方法对幼鼠进行48h的完全性睡眠剥夺，分别在睡眠剥夺前、剥夺后24h及48h后采用Morris水迷宫对幼鼠的学习记忆进行测试，之后处死并采用基因芯片技术对幼鼠脑内基因谱表达进行研究，2组幼鼠基因表达差异〉2．0或者〈0．5的被定义为差异有显著性。结果完全性睡眠剥夺48h后，实验组幼鼠在水迷宫第1轮次测试中从第3个入水点入水找到平台的路程[（499．57±92．63）cm]与时问：（29．83±6．26）s]均长于对照组[分别为（283．84±20．40）cm，（15．33±0．95）s]，差异均有显著性（P〈0．01），第4个入水点实验组找到平台的路程[（1042．51±367）cm]与时间[（38．17±8．97）s]均长于对照组：分别是（489．02±160．38）cm，（20．00±4．52）s]，均差异有显著性（P〈0．01）：完全性睡眠剥夺后48h幼鼠大脑中有11个与学习记忆相关的基因出现显著变化。结论幼鼠在完全性睡眠剥夺后出现的记忆受损形式与成年大鼠不同，其空间参考记忆维持能力受损可能与脑内影响突触传递的基因变化有关。
Objective To explore the effect of total sleep deprivation on learning and memory ability of young rats and to find out some clues of its mechanism. Methods 12 weaned （3-week-old） male Spraque-Dawley rats were randomly divided into sleep deprivation（SD） group and control group （CC）. SD was introduced by ＂Flower pot＂ technique, and lasted for 48 hours. The Morris water maze was used to test the spatial learning and memory of young rats before SD and after SD 24h,SD 48h. All the rats were decapitated after 48b SD, and the cerebral cortex and hippocampus were dissected for eDNA microarray containing 1176 cDNA. The genes whose rations （SD/CC） were 〉2.0 or 〈 0.5 were considered significant different between two groups. Results The impairment of retention of spatial reference memory was showed in SD group after 48 hours total sleep deprivation, while memory acquisition was not effected by 48b SD. The differences were not found in the spatial learning and working memory between SD and CC group. Among 85 genes which were screened out with 40 upregulated and 45 downregulated, there were 11 genes related to learning and memory. The upregulated genes were Rasal, Fgf2, Bid3 and Gnao, and the downregulated were Cd9,Fgfl ,Chrna6 and four glutamate receptors including Grin2b, Grial, Gria4 and Grml. Conclusion Compared with the effect of short term total sleep deprivation on adult rats, the impairment of memory retention after 48 hours sleep deprivation was newly found, and the changes of synaptic conduction related gene expression may be contribute to the mechanism of this memory impariment.
出处
《中国行为医学科学》
CSCD
2008年第11期972-975,共4页
Chinese Journal of Behavioral Medical Science
基金
基金项目：国家自然科学基金（30500410）
上海市重点实验室（04DZ05904）
上海市儿科学重点学科（T0204）
上海市医学重点学科（05Ⅲ002）
关键词
睡眠剥夺
幼鼠
学习记忆
基因芯片
Sleep deprivation
Young rat
Learning and memory
eDNA mircoarray
