摘要
目的观察宫颈癌HeLa细胞chk1/2基因表达改变对X射线诱导凋亡的影响。方法以脂质体为载体将chk1/2正义链和反义链转染HeLa细胞,用Western blot检测HeLa细胞中chk1/2蛋白的表达,用流式细胞仪和TUNEL法检测X射线照射后细胞凋亡率。结果转染chk1/2反义寡核苷酸可明显抑制chk1/2蛋白表达(F=22.69,P<0.05),X射线作用下细胞凋亡率明显高于转染正义链组(F=23.65,P<0.05)。结论反义封闭chk1/2基因可增加HeLa细胞对X射线照射的凋亡敏感性。
Objective To investigate influence of alteration in enpression of checkpoint kinase1/2 （chk1/2）on induced X-ray apoptosis of cervical cancer cells HeLa. Methods The positive - sense and anti-sense strands of oligonucleotide were transfected into HeLa cells through vector liposome. The expression of chk1/2 was detected by using Western blot method. The apoptosis rate of HeLa cells after X-ray radiation was detected by flow cytometer and TUNEL method. Results Transfection with antisense oligonuleotide of chk1/2 inhibited expression of chk1/2 protein in the Lela cells （ F = 22.69, P 〈 0.05 ）, and the apoptosis rate after X-ray radiation was significantly higher than that of the positive-sense oligonuleotide transfection group （ F = 23.65, P 〈 0. 05 ）. Conclusion Antisense blocking of chk1/2 gene could increase apoptostic sensitivity of HeLa cells to X-ray radiation.
出处
《中国妇幼健康研究》
2008年第6期549-551,644,共4页
Chinese Journal of Woman and Child Health Research
基金
陕西省科技计划资助项目(2006K09-G11-3)
