摘要
目的用FRNK表达质粒瞬时转染FN诱导的HSCs,探讨FRNK对HSCs增殖的影响。方法在脂质体介导下用FRNK表达质粒瞬时转染HSCs,用改良的MTT技术测定细胞增殖,用Western blot及RT-PCR技术检测各指标蛋白及mRNA表达。结果与nFRNK组相比,FRNK在FN诱导的HSCs中大量表达后,于12、24和48h的增殖抑制率分别为20.07%、26.16%和29.77%(P<0.01);FRNK抑制FAK磷酸化和ERK1、p-ERK的表达,而FN则促进FAK、ERK1和p-ERK表达。结论在脂质体介导下瞬时转染FRNK表达质粒,可时间依赖性的抑制HSCs增殖;FAK-ERK信号传导通路可能参与了该过程。
Objective To investigate the effect and mechanism of FRNK on the proliferation of Hepatic Stellate Cells in vitro. Methods FRNK plasmid mediated by cationic liposome was transfected into HSCs. The proliferation of HSCs was evaluated by modified MTT assay. The level of FRNK, FAK, p-FAK （Tyr397）, ERK1 and p-ERK in HSCs were assayed by Western blot and RT-PCR. Results Compared with nFRNK plasmid group, FRNK inhibited the proliferation of HSCs and the inhibition rates at 12,24 and 48 h were 20. 07%, 26. 16% and 29. 77% （P 〈0. 01） respectively. Compared with the nFRNK plasmid group, the expression of p-FAK, ERK1 and p-ERK in FRNK plas- mid group reduced; However, as compared with the control group, the expression of p-FAK, ERK1 and p-ERK in FN group increased. Conclusion FRNK can inhibit the HSCs proliferation in a time-dependent manner and FAK-ERK signal transduction pathway was perhaps involved in the process.
出处
《基础医学与临床》
CSCD
北大核心
2008年第11期1151-1155,共5页
Basic and Clinical Medicine
基金
河北省自然科学基金(301361)
河北省卫生厅资助项目(2003055)
河北省普通高校强势特色学科专项经费
关键词
肝星状细胞
增殖
黏着斑激酶相关非激酶
黏着斑激酶
细胞外信号调节激酶
hepatic stellate cell
proliferation
FAK related non-kinase
focal adhesion kinase
extraeellular signal-regulated kinase
