摘要
通过ACC筛选法在玉米根部提取细菌CR2和CS2,并用其菌体悬浮液对玉米种子进行浸泡处理后,种植于0.4%和0.8%NaCl溶液胁迫下的珍珠岩—草炭土栽培载体中,20天后测量;实验表明,在0.8%NaCl溶液的胁迫下,用CR2和CS2浸种的玉米株高、根长、干重和过氧化物酶活力、可溶性蛋白含量、脯氨酸含量、叶绿素含量这几项指标变化比较明显.这说明,促生菌CR2和CS2在一定程度上提高了玉米的抗盐能力.
It extracts bacteria CR2 and CS2 from the root of corn through ACC screening mothed, and were treated by soaking with their cell suspension, then, planted in the peat mixed perlite which was on the stress of 0.4%and 0.8% NaCl liquor, twenty days latter, measuring. The results showed that,on the stress of 0.4% NaCl liquor, every target of corn which was soaking with CR2 and CS2 is not significant; On the stress of 0.8% NaCl liquor, the root length of com plants soaking with CR2 improved 11.7 %, the dry matter accumulation of the below ground part improved 23.23 %, proline content decreased 14.10%, chlorophyll content improved 35.60% ;The root length of com plants soaking with CS2 improved 17.3 %, the dry matter accumulation of the below ground part improved 63.22%, peroxidase activity improved 38.45 %, proline content declined 20.82%, chlorophyll content improved 28.40%. It showed that, to a certain extent PGPR CR2 and CS2 improved the adaptation to salt of com.
出处
《吉林师范大学学报(自然科学版)》
2008年第4期4-7,共4页
Journal of Jilin Normal University:Natural Science Edition
基金
吉林省教育厅科研项目(吉教科合字2005第78号)