摘要
目的:建立测定草履虫静脉麻醉药半数麻醉有效浓度(ED50)的方法。方法:139只运动状态良好的草履虫纳入本研究。以加药后4min内虫体出现沿自身长轴的高速自转运动,维持时间长于1min且在毛细玻璃管长轴的位移小于1cm为终点指标。采用序贯法,通过把虫体置入含不同浓度氯胺酮的缓冲液中,用Dixon-Mood法计算出ED50。重复实验六次。观察48h后虫体的存活情况。结果:六次实验测量草履虫的运动速度比较无统计学差异(P>0.05)。六次实验测定的ED50值分别为1.517±0.207、1.600±0.226、1.800±0.055、1.650±0.245、1.700±0.105、1.417±0.175g/L,无统计学差异(P>0.05)。48h后虫体均存活。结论:结果提示毛细玻璃管法稳定性较好,是一种可行的测定草履虫静脉麻醉药ED50的方法。
Objective：To establish a method of determining paramecium ED50 of intravenous anesthetics.Methods：Total 139 paramecia subjects were included in the study.The up-and-down sequential allocation technique was used to determine ED50 of ketamine in the appearance of high speed rotation more than one minutes and move less than one centimeter in glass capillairy.Calculation of ED50 was determined using Dixon-Mood method.The experiment was repeated for six times.Survival rate was observed 48 hours later.Results：There was no significant difference in the speed of paramecium at the six experiments.ED50 was 1.517±0.207,1.600±0.226,1.800±0.055,1.650±0.245,1.700±0.105 and 1.417±0.175 g/L respectively.There was no significant difference in ED50（P〉0.05）.All paramecia were alive in 48 hours.Conclusion：Our results suggest that this glass capillary method is useful for determining paramecium ED50 of intravenous anesthetics.
出处
《华西医学》
CAS
2008年第4期816-817,共2页
West China Medical Journal
