摘要
从无锡市某硫酸厂采集的土样中分离到一株具有硫化物氧化能力的细菌,对该细菌的16S rDNA序列进行测定,并根据16S rDNA构建了系统发育树。结果表明,TL-1与硫杆菌属有较高的同源性,与那不勒斯硫杆菌(Thiobacillus neapolitanus)同源性为95.3%,结合菌落、细菌形态鉴定菌株为硫杆菌属(Thiobacillus),并命名为TL-1。同时,对TL-1的脱硫效率进行了初步研究。结果表明,在硫化物为惟一硫源且其质量浓度分别为60,120,180,240 mg/L时,4 h时硫化物去除率分别达到87.92%、71.25%、63.45%和13.31%。
A sulfide-oxidizing strain TL-1 was screened and further identified. It was found that the 16S rDNA sequence of the strain was most closely to that of the Thiobacillus neapolitanus with the similarity of 95.3%. Moreover a phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relative bacteria species which was selected based on the colony and cell morphology observation. The screened strain was identified to belong to the species of Thiobacillus. Furthermore, when 60, 120, 180, 240 mg/L of sodium sulfide were used as the sole sulfur source, the removal efficiency reached at 87.92%, 71. 25%, 63.45%, 13.31% after 4 h of desulfurization process, respectively.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第5期113-116,共4页
Journal of Food Science and Biotechnology
基金
江苏省高技术研究项目(DG2006044)
江苏省自然科学基金项目(BK2006023)
关键词
硫化物氧化菌
鉴定
16SrDNA
脱硫能力
sulfide-oxidizing microorganism
identification
16S rDNA
removal efficiency
