摘要
采用水培法，研究了油菜湘杂油二号（耐Al型）和浙双758（Al敏感型）的生物量、根尖Al含量及叶片保护酶活性、叶片MDA和Pro含量对Al胁迫的响应。结果表明，胁迫7d时，湘杂油二号根系、地上部和总生物量下降率（13％～36％、13％～36％、5％～17％）均小于浙双758（30％～53％、12％～20％、16％～28％）。胁迫14d生物量下降比胁迫7d时明显，根冠比也随Al^3＋浓度增加和时间延长而下降；同时，株高变化趋势与生物量变化趋势基本一致。胁迫7d下，湘杂油二号根尖A1含量在3．94～5．88μg·g^-1之间，浙双758在4．96—7．54μg·g^-1之间，胁迫14d时根尖Al积累进一步增加。Al^3＋对叶片SOD、CAT和POD具有激活效应，SOD上升幅度较为明显，胁迫7d和14d时，200μmol·L^-1。Al^3＋浓度下湘杂油二号分别增加2．50倍和2．03倍，浙双758分别增加2．02倍和1．83倍，Al^3＋对CAT和POD的诱导效应小于SOD；从时间差异看，SOD、CAT的绝对活性和增幅在胁迫14d时小于胁迫7d，POD相反。叶片MDA和脯氨酸含量均随Al^3＋浓度增加和时间延长而升高，MDA含量在湘杂油二号中低于浙双758，而脯氨酸含量是湘杂油二号高于浙双758。研究认为，湘杂油二号根尖排斥A1的能力较强，且叶片能够维持较高的保护酶活性和较高的脯氨酸含量，因而其耐Al性强于浙双758。
A solution culture was eondueted to study the effeet of aluminum （Al）on a tolerant genotype （XZY2）and a sensitive genotype （ZS758）of oilseed rape. The biomass growths, Al-aecumulations, aetivities of proteetive enzymes and contents of MDA and proline in leaves of oilseed rape seedlings were investigated. The results showed, after being treated 7 d, the dry weight of root and shoot and biomass of XZY2 decreased significantly by 13%-36%, 13%-36% and 5%-17%, the corresponding decrease for ZS758 were 30%-53%, 12%-20% and 16% -28%, respectively. The decrease of biomass was more significant in 7 d treatment than in 14 d treatment and the ratio of root/shoot decreased with the increasing Al^3＋ in euhure solution and treated time and the plant height deereased simultaneously with the same trends. Al eontents in roots of both varieties inereased under A1 stress, whieh were much higher in Al-sensitive genotypes XZY2 （3.94-5.88 μg·g^-1）than in Al-tolerant ZS758 （4.96-7.54 μg·g^-1）in 7 d treatments, and were even higher after being treated for 14 d. The protective enzymes were aetivated by A1, especially the SOD, after being treated by 200 μmol·L^-1 Al^3＋ for 7 d and 14 d, the SOD activities inereased 2.50 times and 2.03 times in XZY2 and 2.02 times and 1.83 times in ZS758, respectively;In contrast to SOD, CAT and POD were less affected by Al stress. In addition, compared to 7 d treatment, the activities of SOD and CAT decreased but that of POD inereased after 14 d treatment. With the inereased Al^3＋ eoucentrations in culture media, the content of MDA in leaves was lower in the XZY2 than in ZS758, while higher content of proline were observed in XZY2. It suggested that toleranee of XZY2 to A1 than ZS758 was partially aehieved by excluding Al from root tip and inereasing the protective enzymes activity or proline contents in leaves of oilseed rape.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第6期2351-2356,共6页
Journal of Agro-Environment Science
基金
国家自然科学基金(30540056)
浙江省自然科学基金(504135)
关键词
AL
胁迫
油菜
生物量
保护酶
aluminum
stress
oilseed rape（Brassica napus L. ）
biomass
protective enzyme
