摘要
镉是一种主要的环境污染物,它可以通过在作物体内的积累而进入人类的食物中,但是,对于Cd2+的生理效应与毒害机理的了解并不十分清楚,而从蛋白质组角度分析Cd2+害机理文献更少。本文以中国白菜为研究材料,采用室内培养方法,研究了10、30、50、100、1000μmol·L-1镉浓度处理对白菜幼苗生长受害及与蛋白质组变化的关系。结果表明,低浓度镉(10、30μmol·L-1)对幼苗生长无明显有害影响,但在50μmol·L-1镉浓度下,幼苗高度、胚根长度、鲜重、干重均显著下降,证明50μmol·L-1是白菜忍耐镉害的临界浓度。实验同时发现,随着镉处理浓度的提高,幼苗生长受害加重,叶绿素含量变化也得出类似的结果。2D-PAGE技术分析则表明,经50μmol·L-1镉处理后的胚根、胚轴、叶片蛋白质组产生明显变化,胚根中诱导出13个新的蛋白质斑点,7个蛋白质斑点消失,胚轴中有11个蛋白质斑点消失;叶片中诱导出17个新的蛋白质斑点,7个蛋白质斑点消失。很明显,白菜对镉的胁迫反应涉及到多个蛋白质(酶)的参与。
Cd^2＋ is a major environmental pollutant that enters human food via accumulation in crops, but the biological effects of this metal, the mechanism of its toxicity are not yet clearly understood, and little information is known about stress-elicited changes in plants at the proteome level. In present study, we used Chinese cabbage as experimental materials, investigated the effects of Cd^2＋ on growth and proteome changes of seedlings treated with 10-1 000 μmol·L^-1. We also analyzed the relationship of seedling growth inhibition and proteome changes. The results indicated that 10 μmol·L^-1 and 30 μmol·L^-1 Cd^2＋ did not inhibit the seedling growth, but the severe toxicity damages were observed in seedlings treated with 50μmol·L^-1 Cd^2＋, with seedling height, root length, fresh weight and dry weight decreasing. The results showed that 50μmol·L^-1 was the threshold concentration of Chinese cabbage seedlings tolerance to Cd^2＋. Seedling growth inhibition increased with an increase in the concentration of Cd^2＋ from 50 μmol·L^-1 to 1 000 μmol·L^-1. Similar experimental results were observed in chlorophyll content changes of seedlings treated with different concentrations of Cd^2＋. Using two-dimensional gel electrophoresis, several protein changes were observed in seedlings treated with 50 μmol·L^-1 Cd^2＋, 13 new protein spots were induced and 7 protein spots were lost in root, 11 protein spots were lost in hypocotyls and 17 new protein spots were induced, 7 protein spots were lost in leaves, indicating that several proteins（enzymes ）were involved in the response of Chinese cabbage to Cd^2＋ stress.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第6期2345-2350,共6页
Journal of Agro-Environment Science
基金
教育部科学技术研究重点项目(02010)
关键词
镉
临界剂量
蛋白质组
双向凝胶电泳
白菜
cadmium
threshold concentration
proteome
two-dimensional gel electrophoresis
Chinese cabbage
