摘要
对中国大陆地区分布的野猪亚种分类尚存在争议。本研究通过对野猪11个微卫星位点的变异分析,探讨了中国大陆地区野猪的遗传结构,以期对野猪亚种分类问题有所启迪。对野猪华北、华南和东北种群的分析表明,各种群基因库中都维持了较高的遗传变异水平。基于遗传距离构建的系统树分析发现,研究中所涉及的所有亚种在系统树中混杂,没有任何亚种在其中构成显著的支系。基于微卫星变异的FCA分析不能区分华南、华北、东北三个种群。基于Fst检验的遗传差异分析发现,长江两侧的华南、华北种群之间的遗传差异较小(Fst=0.014),表明长江两侧的野猪可能存在着较高水平的基因流,长江并非是一个有效的空间隔离;东北地区野猪和华北、华南地区野猪之间的遗传差异相对较大(Fst=0.040、0.042),东北野猪可以作为独立的亚种S.s.ussuricus。
In order to discuss the subspecific classification of wild boar Sus scrofa in China, we studied their genetic structure using 11 microsatellites, with emphasis on South China, North China and Northeast China. High levels of genetic variation were detected in South China （SC）, North China （NC） and Northeast China （NE） wild boar populations. This was consistent with the huge population size of the wild boar and its status as a widely-distributed species. Weak genetic divergence was observed in all subspecies involved in this study. Individuals from all subspecies were highly mixed in the phylogenetic tree based on the allele sharing distance （DAS）, no distinct clade was found according to the defined subspecies. Even considering the two main factors, which reflect the biggest genetic differentiation among populations, it is impossible to distinguish SC, NC and NE from each other by FCA （Factor Correspondence Analysis）. Using the F-statistic test, weak genetic differentiation （F,t = 0.014） was detected between SC and NC, which indicated a high gene flow between two sides of the Yangtze river. The Yangtze River is an insurmountablegeographical barrier for wild boar. The stronger population differentiations were observed between NE and NC population, this may suggest that the wild boars in NE area could be defined as the subspecies S.s. ussuricus
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2008年第5期753-761,共9页
ACTA ZOOLOGICA SINICA
基金
江苏省生物多样性与生物技术重点实验室基金资助~~
关键词
野猪
亚种
微卫星
遗传结构
亚种分化
Wild boar, Sus scrofa, Microsatellite, Genetic structure, Subspecies divergence
