摘要
本文应用抗粒细胞集落刺激因子（G─CSF）的单克隆抗体、采用间接免疫吸附法对148例足月新生儿脐血清中G─CSF水平进行了测定，并与41例正常小儿及50例成人血清相比较，同时观察了G─CSF水平与其性别、出生体重、白细胞总数、中性粒细胞绝对计数的关系。结果显示11例（7．4％）脐血清G─CSF水平低于0．5ng／ml，137例（89％）G─CSF均高于0．5ng／ml，其平均值为3．4ng士0.05ng／ml，而且G─CSF水平与其性别、出生体重、白细胞总数及中性粒细胞绝对计数无明显相关（P均＞0.5）；结果还显示1～5个月小儿（41例）及成人血清（50例）G─CSF水平皆低于0．5ng／ml，提示足月新生儿脐血清G─CSF水平大大高于正常小儿及成人水平，本文首次报道中国足月新生儿脐血清G─CSF正常值，为研究新生儿免疫功能及为脐血的合理应用提供了依据。
We measured the serum levels of granulocyte colony--stimulating factor (G--CSF) in thecord boold of 148 normal full--term neonates immediately after birth by enzyme--linked irnmunosorbent assay (ELLSA) for G--CSF and observed the ralationships between the G--CSFlevels and sex, weight of birth, white blood cell count, absolute granulocyte number. 11 outof 148 cases examined (7. 4% ) showed the G--CSF levels below 0. 5ng/ml, in the 137 cases(92. 6% ), the G--CSF levels more than 0. 5ng/ml, the average value were 3. 4± 0. 05ng/ml),wherease the G--CSF levels of normal adults (50 cases) and healthy children (41 cases) werebelow 0. 5ng/ml, which had statistical singificant (P〈O. 001). The relationships between GCSF levels and sex, weight of birth, white blood cell count, absolute granulocyte numberwere not observed (P＞0. 5). We first reported the normal value of G--CSF in cord blood ofnormal full--term neonates in China, which has important significant on sutdies the immunological function of neonates and rational use resurrn of cord blood.
出处
《中国小儿血液》
1997年第2期73-74,65,共3页
China Child Blood
