摘要
目的:观察蛋白激酶C(PKC)激活后哮喘大鼠气道平滑肌细胞(ASMCs)周期蛋白D1(cyclin D1)的表达变化及两者表达相关性,探讨cyclin D1在PKC调控哮喘大鼠ASMCs增殖中所起的作用。方法:卵清蛋白(OVA)吸入制备SD大鼠2周哮喘模型,原代培养ASMCs。采用正常大鼠(N组)和模型大鼠(A组)第3-6代细胞,分别应用PKC特异性激活剂12-肉豆蔻酰-13-乙酸佛波酯(PMA)及抑制剂Ro-31-8220干预ASMCs,根据不同处理分为4组:(1)空白组;(2)10nmol/L PMA组;(3)10nmol/L PMA+5μmol/L Ro-31-8220组;(4)5μmol/L Ro-31-8220组。采用流式细胞术,四甲基偶氮唑盐(MTT)法,增殖细胞核抗原(PCNA)染色等方法观察药物对ASMCs增殖的影响。RT-PCR方法检测PKC-α和cyclin D1 mRNA表达水平,Western blotting方法检测PKC-α和cyclin D1蛋白表达水平。线性相关分析评价PKC-α和cyclin D1表达水平的关系。结果:(1)在哮喘组,与空白对照比较,PMA组、Ro-31-8220组S+G2/M期比例、吸光度A值、PCNA阳性表达率,差异显著(P<0.01)。在正常组,与空白对照相比,PMA组、Ro-31-8220组S+G2/M期比例、吸光度A值、PCNA阳性表达率差异均显著(P<0.01)。(2)哮喘组内PMA组、Ro-31-8220组PKC-α以及cyclin D1 mRNA和蛋白表达水平与空白对照比较,差异显著(P<0.01)。正常组和哮喘组变化趋势一致。(3)在mRNA水平,大鼠ASMCs PKC-α和cyclin D1的表达呈明显正相关(r=0.476,P<0.05),在蛋白水平两者的表达亦呈明显正相关(r=0.899,P<0.01)。结论:PKC-α能促进正常大鼠和哮喘大鼠ASMCs增殖,其在基因和蛋白的表达水平与cyclin D1的表达呈正相关,提示PKC-α可能通过调节cyclin D1而影响ASMCs的增殖。
AIM： To observe the expression of cyclin D1 following PKC activation and the correlation between PKC -α and cyclin D1 in asthmatic rat airway smooth muscle cells （ASMCs）, and to discuss the effects of cyclin D1 on the proliferation of airway smooth muscle cells regulated by PKC in asthmatic rat. METHODS ： Twelve SD rats were randomly divided into control group （ group N） and asthmatic 2 - week group （ group A）, and then subdivided into 4 groups based on the drug interventions ： （ 1 ） control group; （ 2 ） PMA ; （ 3 ） PMA ＋ 5 μmol/L Ro - 31 - 8220 group ; （ 4 ） 5 μmol/L Ro - 31 - 8220 group. The proliferations of ASMCss were examined with cell cycle analysis, MTT colorimetric assay and proliferating cell nuclear antigen （PCNA） immunocytochemical staining. The expressions of PKC -α and cyclin D1 were analyzed by RT - PCR and Western blotting. The data were subject to correlation analysis. RESULTS ： （ 1 ） Compared to group A1, there were significant differences in the percentage of S ＋ G2/M phase, absorbance A value of MTT and the rate of positive expression of PCNA protein in group A2 and A4 （P 〈0.01 ）. The same tendency in group N was observed according with group A. （2） Compared with A1, the ratios of A values of PKC - α mRNA and protein in group A2 and A4 were significantly changed （P 〈 0. 01 ） as well as the ratios of A values of cyclin D1 mRNA and protein. （3） There were positive correlations between PKC -α and cyclin D1 in mRNA （r = 0. 476, P 〈 0.05） and protein（ r = 0. 899, P〈0. 01）. CONCLUSION： The activation of PKC -α promotes ASMCs proliferation in asthmatic rats, and there are positive correlations between the PKC - α and cyclin D1 in mRNA and protein, indicating that the PKC - α signal pathway may be involved in the process of airway smooth muscle remodeling by regulating the expression of cyclin D1 in asthmatic rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第11期2214-2219,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30670925)
