摘要
[目的]培养黄芩优良品种并进行克隆化快速繁育。[方法]用黄芩(Scutellaria baicalensisGeorgi)无菌根作为外植体进行愈伤组织的诱导、分化,黄芩试管苗的继代扩大繁殖、生根等一系列优化试验。[结果]MS+2.0 mg/L 6-BA+0.2 mg/L NAA培养基进行愈伤组织的分化培养效果最好;MS+2.0 mg/L 6-BA+0.2 mg/L NAA进行试管苗的复壮效果较好;最有效的生根培养基为1/2 MS+0.3mg/L NAA,生根率可达96.7%。[结论]采用单节培养的方法,可以大幅度提高黄芩繁殖速度。
[ Objective ] The research aimed to culture the fine varieties of Scutellaria baicalensis Georgi and carry out cloning rapid- propagation. [ Method ] Research by sterile root explants of S. baicalensis Georgi including callus inducing, differentiation, planflet propagation and rooting were designed. [ Result] The results showed that MS ＋ 2.0 mg/L 6-BA ＋ 0.2 mg/L NAA was the best medium for callus differentiation and plantlet rejuvenation. The best medium was 1/2 MS ＋ 0.3 mg/L NAA for rooting , and the rooting rate reached 96.7% . [ Conclusion] The !oropagation rate could be improved using single node for S. baicalensis Georgi.
出处
《安徽农业科学》
CAS
北大核心
2008年第29期12600-12601,12621,共3页
Journal of Anhui Agricultural Sciences
基金
黑龙江省骨干教师项目
关键词
黄芩
愈伤组织
组织培养
快速繁殖
Scutellaria baicalensis Georgi
Callus
Tissue culture
Rapid-propagation
