摘要
为了探讨AgNO3在离体培养物形态发生中的作用,以金水梨组培苗为外植体,研究了不同浓度AgNO3对梨叶片再生不定芽过程中内源多胺含量的影响。结果表明,多胺含量会随着外植体细胞分裂分化迅速增加,其中腐胺含量在培养5d时形成高峰,精胺在20d时出现峰值,而亚精胺在培养过程中2次出现峰值。经AgNO3处理后叶片内源多胺含量与对照相比明显增多,其中以0.1mg/LAgNO3处理效果最明显。
Effects of different AgNO3 concentrations on the content of endogenous polyamine in in vitro leaves of Pyrus brestchneideri Rehd. cv. Jinshui was studied in order to understand the effect of AgNO3 in the process of in vitro morphologenesis. The results indicated that the content of endogenous polyamine would be enriched with cell division and differentiation. The content of putrescine （Put） and spennine （Spm） formed pinnacles on 5th day and 20th day respectively, while the content of spermindine （Spd） was enhanced on both 10th and 20th day. It could be drawn the conclusion that Put, Spm and Spd regulated the leaf cell division and differentiation alone or together. The content of endogenous polyamine in leaves treated with AgNO3 was much higher than that treated without AgNO3. Among the three treatments with AgNO3 tested, the treatment with 0.1 mg/L AgNO3 stimulated more endogenous polyamine formation than the others.
出处
《果树学报》
CAS
CSCD
北大核心
2008年第6期920-923,F0004,共5页
Journal of Fruit Science
基金
国家自然科学基金“西南地区树莓植物遗传多样性研究与特异资源筛选”(30671451)
教育部“新世纪优秀人才支持计划”(NCET-04-0905)
高等学校全国百篇优秀博士学位论文作者专项基金(200253)
关键词
梨
叶片培养
AGNO3
内源多胺
Pear （Pyrus spp.）
Leaf culture
AgNO3
Endogenous polyamines
