摘要
目的:探讨ADAM23基因mRNA及蛋白在结直肠癌中的表达规律及其临床意义.方法:我院2007-03/2008-03手术治疗的结直肠癌患者45例,分别取结直肠癌组织标本以及配对癌旁组织标本,应用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学方法分析患者ADAM23基因mRNA及蛋白的表达进行.结果:结直肠癌组织中ADAM23基因mRNA及蛋白的表达明显降低(37.8%vs95.6%,28.9%vs86.7%,均P<0.01),且其表达与患者的年龄、性别以及肿瘤的大小无关,而与结直肠癌的分化程度、浸润深度、淋巴结转移和临床分期关系密切(χ2=5.688,14.79,11.8172,11.8172,P<0.01或0.05).结论:ADAM23参与了结直肠癌的发展过程,并与结直肠癌的部分生物学行为有关,可能成为结直肠癌诊断和判断其生物学特性的重要的分子生物学检测指标.
AIM： To investigate the expression of ADAM23 mRNA and protein in human colorectal cancer and its clinical significance. METHODS： Forty-five cases with colorectal cancer undergoing surgical treatment in our hospital from 2007 to 2008 were collected. Reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry were performed to detect the expression of ADAM23 mRNA and protein respectively in tumor and paratumorous tissues. RESULTS： The expression levels of ADAM23 mRNA and protein were significantly lower in tumor tissues than those in paratumorous tissues （37.8% vs 95.6%, 28.9% vs 86.7%; both P 〈 0.01）. ADAM23 expression had no correlation with patients＇ age, sex and tumor size, but markedly related to tumor differentiation degree, infiltration depth, lymph node metastasis and clinical stages （Х^2 = 5.688, 14.79, 11.8172, 11.8172; P 〈 0.01 or 0.05）. CONCLUSION： ADAM23, which is associated with partial biological behaviors, may be involved in the development of colorectal cancer, and it may serve as an important molecular biological indicator in diagnosing and predicting the biological behaviors of colorectal cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第30期3406-3409,共4页
World Chinese Journal of Digestology
基金
黑龙江省科技攻关资金资助项目
No.GC06C416-01~~
