摘要
设计1对克隆引物,从兔十二指肠黏膜组织提取总RNA,进行RT-PCR,将PCR产物与pMD19-T载体连接后转化E.coliJM109感受态细胞,检测阳性克隆、测序并进行序列分析。结果表明:克隆的兔CCR9基因长为624 bp,编码由208个氨基酸残基组成的CCR9前体蛋白,预测CCR9具有多个跨膜区。克隆的兔CCR9基因与绵羊、人的同源性分别为82.9%、82.7%;推导的兔CCR9氨基酸序列与绵羊、人的同源性分别为80.1%、82.2%,其结构特征与绵羊、人的相一致。
A pair of cloning primers were designed, total RNA was extracted from the duodenum mucosal tissue of Oryaolagus curticulus, and mRNA sequence was amplified by RT- PCR. The PCR products were ligated into the pMD19-T vector, and then transformed into competent cells of E. coli JM109. The positive clone was identified and the sequence was tested and analyzed. The results indicate that gene CCR9 in Oryctolagus cuniculus has the length of 624 bp and encodes 208 amino acids. Tertiary structure predicts that CCR9 contains several domains. Identity analysis shows that the CCR9 nucleotide sequence in Oryctolagus cuniculus shares 82.9%, 82. 7% homology with that of Ovis aries and Homo sapiens respectively, and the predicted amino acid sequence of CCR9 in Oryctolagus cuniculus shares 80.1%, 82.2% homology with that of Ovis aries, Homo sapiens respectively, revealing that the structural feature of the former CCR9 is consistent with the latter two CCR9s.
出处
《江西农业学报》
CAS
2008年第10期1-3,13,共4页
Acta Agriculturae Jiangxi
基金
国家自然科学基金(30671531)
关键词
兔
CCR9基因
克隆
序列分析
Oryctolagus cuniculus
Gene CCR9
Molecular cloning
Sequence analysis
