摘要
探讨钙依赖性上皮细胞粘附分子Ｅ－钙粘附素（Ｅ－ＣＤ）的表达和胃癌生物学行为的关系。方法应用流式细胞术和细胞免疫荧光技术，定量检测了８０例手术切除、石蜡包埋的胃癌标本中Ｅ－ＣＤ表达和ＤＮＡ含量，并对切除胃标本做系统病理学检查。全部病例均随访５年以上。结果胃癌组织Ｅ－ＣＤ表达量（ＦＩ值）为０．６７±０．１１，正常胃粘膜表达量为１．００±０．０７，两者差异有非常显著意义（Ｐ＜０．００１）。２例早期胃癌Ｅ－ＣＤ表达亦降低。Ｅ－ＣＤ表达在Ｂｏｒｍａｎ４型、弥漫生长、未分化型、淋巴结多数转移（５个以上）或转移至Ⅱ站以远者、浸透浆膜及生存在５年以内的胃癌中降低更明显（Ｐ＜０．００１）。异倍体胃癌Ｅ－ＣＤ表达量及阳性表达率均较二倍体胃癌低（Ｐ＜０．０１）。Ｅ－ＣＤ阴性表达胃癌ＤＮＡ指数（ＤＩ）值和增殖指数（ＰＩ）值较阳性表达的高（Ｐ＜０．０１）。结论胃癌组织Ｅ－ＣＤ表达下调与胃癌较恶的生物学行为及预后不佳密切相关，并发生于胃癌的早期阶段。Ｅ－ＣＤ定量分析对判断胃癌淋巴结转移程度有一定价值。
Objective To evaluate the relationship of E cadherin (E CD) expression to cellular DNA content and biological behavior of gastric cancer. Methods E CD expression and cellular DNA content were quantitatively measured by flow cytometry and immunofluorescence methods in 80 cases of formalinfixed, paraffin embedded gastric cancer. Systematic pathological examinations and follow up were performed for all cases. Results E CD expression was significantly reduced in all cases of gastric cancer. fluorescence Index (FI) of E CD expression was 0.67±0.11 in gastric cancer, 1.0±0.07 in normal gastric mucosa ( P <0.001). The reduction of E CD expression was also found in 2 cases of early gastric cancer. Tumors with a decrease in E CD expression occurred significantly more frequently in undifferentiated, diffuse growth pattern Borrmann 4 type, positive lymph node (LN) metastasis and infiltrated serosa type gastric cancer, of which survival time was within 5 years ( P <0.001). E CD expression was also reduced in gastric cancer with the number of LN metastasis above 5, metastasis to more than group 2. E CD exprression was lower in uneuploid cancer than that in diploid cancer ( P <0.01). The value of DI and PI with negative E CD expression was significantly higher than that of positive E CD expression ( P <0.01). Conclusion Down regulation of E CD expression correlates with bad biological behavior and poor prognosis of gastric cancer. The reduction of E CD expression may take place during early time of gastric cancer. Quantitative analysis of E CD expression may have some value in evaluating the intensity of LN metastasis of gastric cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第9期668-671,共4页
National Medical Journal of China
