摘要
目的探讨长期失喉返神经支配后．人环杓后肌胶质细胞源性神经营养因子（glial cell line—derived neurotrophie factor，GDNF）及其受体GDNFR-α1的变化规律。方法38例不同时限喉返神经损伤的患者，按神经损伤时限归入0．5～年、1～年、2～年、≥3年组，对照组12例，为因喉癌行喉全切除术且肿瘤未侵及环杓后肌者。采用免疫荧光双标记法分别标记GDNF和GDNFRα1，运用图像分析系统对环杓后肌GDNF和GDNFRα1表达变化进行评估。结果失神经0．5～年组、1～年组环杓后肌GDNF和GDNFR-α1表达的平均灰度值和阳性区百分比显著高于其他各组（P〈0．001），失神经0．5～年组明显高于失神经1～年组（P〈0．001）。对照组、失神经2～年组，失神经≥3年组之间无疆著性差异（P〉0．05）。结论失神经支配1年内环杓后肌GDNF和GDNFR—α1表达较高。失神经支配1～2年内环杓后肌GDNF和GDNFR-α1仍有表达，说明失神经支配2年以内喉肌GDNF的功能状态较好。
Objective To investigate the changes of the expression of GDNF and GDNFRal of long-term denervation of posterior cricoarytenoid muscles（PCAMs）. Methods 38 patients with vocal paralysis were grouped into four according to their denervated period of time while 12 normal PCAMs as control group. Using double immunofluorescence stain, changes of GDNF and GDNFR1 expression were observed in myofibers at different time points after denervation. Results Double immunofluorescence stain with antibodies against GDNF and GDNFR1 showed no staining in the control group, and study groups. However, after the muscle denervation lasted for 6-12 months and 1-2 years, noted was a significant accumulation of GDNF and GDNFR1 protein in eytolemma and endochylema of myofiber. The mean grey scales and positive region ratios were compared using the image analysis system. The results revealed the levels of GDNF and GDNFR1 protein expression in 6-12 months group, 1- 2yr group changed significantly （P〈 0. 001）. However, the levels of GDNF and GDNFR1 protein expression in Groups with 2-3 years and more than 3 years and the control group showed no significant changes （P≥0.05）. Conclusion The changes in expression of GDNF and the acceptor GI）NFR-α1,a powerful neurotrophic factor, implied that a good nervous regenerated microenvironment in PCAMs within 2 years. This experiment indicated that denervated posterior cricoarytenoid muscles are able to regain their functions through reinneration within 2 years.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2008年第6期470-472,共3页
Journal of Audiology and Speech Pathology
