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布鲁菌重要毒力调控基因在环境刺激和细胞侵染过程中的转录研究 被引量：2

Transcriptional analysis of Brucella virulence regulation genes under stress conditions and during cell infection
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摘要 目的了解dnaK、htrA、ribR和gntR等布鲁菌的重要毒力调控基因的功能。方法本研究利用定量RT—PCR的方法分析了dnaK、htrA、vjbR和gntR在酸等体外刺激条件下和巨噬细胞感染过程中的转录变化。结果这些毒力调控基因在体外刺激条件下、侵染过程中可特异的诱导表达，但是它们在这些刺激条件和胞内不同时期的转录丰度不同。结论dnaK、htrA、vjbR和gntR在布鲁菌感染过程的不同环节发挥作用。 Objective To study the important virulence regulation genes of Brucella, and to understand their function. Methods Quantitative RT-PCR was used to quantify their relative transcription profiles under stress conditions and during macrophage cell infection. Results These genes were activated at different levels under these conditions and during cell infection, indicating their roles in pathogenesis-at different stage of infection. Conclusion The transcription profiles of these genes have different effects about their functions.
作者 王玉飞 乔凤 钟志军 汪舟佳 杜昕颖 于雅琴 陈泽良 黄留玉
机构地区 军事医学科学院疾病预防控制所 吉林大学公共卫生学院人畜共患病教育部重点实验室
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2008年第10期919-924,共6页 Chinese Journal of Microbiology and Immunology
基金 国家自然科学基金项目（30600024） 国家“863”资助项目（2007AA022412）
关键词 布鲁菌 转录 基因表达 Brucella Transcription Gene expression
分类号 R378 [医药卫生—病原生物学] R516 [医药卫生—内科学]
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参考文献11

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同被引文献19

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  • 9Leyn SA, Kazanov MD, Sernova NV, et al. Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis [J]. J Bacteriol, 2013, 195(11): 2463-2473. doi:10.1128/JB.00140-13.
  • 10Kroger C, Dillon SC, Cameron AD, et al. The transcriptional landscape and small RNAs of Salmonella enterica serovar Typhimurium [J]. Proc Natl Acad Sci U S A, 2012, 109(20): E1277-1286. doi:10.1073/pnas.1201061109.

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中华微生物学和免疫学杂志
2008年 第10期

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