摘要
目的提高血培养阳性率,准确快速作出病原学诊断,指导临床合理应用抗菌药物;评价BacT/Alert 3D全自动血培养仪的临床使用情况。方法用BacT/Alert 3D全自动血培养仪对3728份血液标本进行检测;药敏实验采取K-B纸片法按CLSI规定的标准进行。对不同病原菌检出的时间、阳性率和病原菌种类及耐药性进行统计分析。结果3728份血培养阳性率为6.0%,假阳性率为0.2%,假阴性率为0.4%;共检出222株病原菌;12 h内阳性率为0.89%,18 h内阳性率为2.01%,24 h内阳性率为3.51%;血培养中肠杆菌科细菌占29.7%,对阿米卡星、头孢他啶、环丙沙星、亚胺培南的耐药率分别为13.6%、36.3%、42.4%、3.0%;葡萄球菌属占20.3%,对红霉素、左氧氟沙星、头孢西汀、万古霉素的耐药率分别为75.6%、33.3%、68.9%、0;肠球菌属占14.0%,对红霉素、左氧氟沙星、磷霉素、万古霉素的耐药率分别为96.2%、80.8%、23.1%、0;非发酵杆菌占11.3%,对阿米卡星、头孢他啶、环丙沙星、亚胺培南的耐药率分别是32.0%、52.0%、32.0%、32.0%;真菌占12.6%。结论血培养的病原菌较以前分布范围更广、耐药性更高;BacT/Alert 3D全自动血培养仪可作为快速血培养的重要工具,为临床快速提供诊断帮助,提高培养阳性率,不仅病原菌的检出时间短,检出菌种范围广,而且具有快速、准确等优点。
OBJECTIVE To enhance the positive rate of the blood culture in order to make pathogenic diagnosis actually and quickly and conducting usage of antimicrobial agents in clinic. To value the clinical applied circumstance of BacT/Alert 3D automated blood culture system. METHODS The 3728 blood cultures were detected by BacT/Alert 3D automated blood culture system,and bacterial susceptibility test was conducted on all isolates using Kirby-Bauer methods with CLSI standards. To statistically analyze the examined time, the positive rate and variety and drug resistance for all kinds of pathogens. RESULTS The blood culture positive rate was 6.0% in the 3728 blood cultures with 222 strains of bacteria. The false positive rate was 0. 2% and the false negative rate was 0. 4%. The positive rate of blood culture was 0. 89% in 12 hours, 2. 01% in 18 hours, and 3.51% in 24 hours. Among all the 222 isolates, 29. 7% were Enterobacteriaceae, the drug resistant rates to amikacin, ceftazidime, ciprofloxacin, and imipenem were 13. 6%, 36. 3%, 42. 4%, and 3. 0%, respectively; 20.3% were Staphylococcus, the drug resistant rates to erythromycin, levofloxacin, cefoxitin, and vancomycin were 75. 6%, 33. 3%, 68. 9%, and 0, respectively; 11. 7% were Enterococcus, the drug resistant rates to errythromyein, levofloxacin, fosfomycin, and vancomycin were 96. 2%, 80. 8%, 23. 1%, and 0, respectively; 11.3 % were non-fermented bacilli, the drug resistant rates to amikacin, ceftazidime, ciprofloxacin, and imipenem were 32.0%, 52.0%, 32.0%, and 32.0%, respectively112.6% were fungi. CONCLUSIONS The pathogens in the blood specimens are more wider in distribution and more higher in drug resistance rates than before. BaeT/ Alert 3D automated blood culture system can be an important tool for the blood culture, it can provide the diagnostic help quickly for the clinic and increase the positive rates in blood culture. It can not only shorten the check-up time, but also be more quick and more exact.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第10期1456-1459,共4页
Chinese Journal of Nosocomiology
关键词
血培养
仪器
病原菌
耐药性
Blood culture
Instrument
Pathogens
Drug resistance
