摘要
目的:探讨胃黏膜癌前病变与胃癌组织中Bcl-2、Bax、p16和p53蛋白的表达和意义。方法:用免疫组化法检测Bcl-2、Bax、p16和p53产物在79例胃癌、23例胃黏膜不典型增生和21例肠上皮化生组织中的表达。结果:Bcl-2在胃癌和不典型增生组织中的阳性率分别为73.4%(58/79)和78.2%(18/23),差异无统计学意义,P=0.273;但是显著高于肠上皮化生组织(38.1%,8/21),P<0.01。Bax、p16和p53在胃癌和不典型增生及肠上皮化生组织中的阳性率分别为41.8%(31/79)、69.6%(18/23)、80.9%(17/21)和45.6%(36/79)、65.2%(15/23)、76.1%(16/21)及82.3%(65/79)、39.1%(9/23)、28.6%(6/21),差异有统计学意义,P<0.01。结论:Bcl-2对胃黏膜细胞凋亡有明显的负性调节作用,而抑癌基因Bax表达对Bcl-2的抑制凋亡功能有对抗调节作用;p16基因直接参与细胞生长增殖的负调节,突变的p53基因通过抑制细胞凋亡而参与胃癌的发生。
OBJECTIVE：To investigate the expression and significance of Bcl-2, Bax, p16 and p53 in the gastric cancer, dysplasia and intestinal metaplasia. METHODS： Immunohistochemistry was used to detecte the activity of Bcl-2, Bax, p16 and p53 in the gastric cancer, dysplasia and intestinal metaplasia. RESULTS： No defference in the gastric cancer （73.4%,58/79）and dysplasia（78.2%,18/23） was found in positve expression of Bcl-2, P=0.273, which was remarkable higher than intestinal metaplasia（38.1%,8/21）, P〈0.01. The positve expression of Bax, p16 and p53 in gastric cancer, dysplasia and intestinal metaplasia was respectively 41.8%（31/79）, 69.6%（18/23）, 80.9%（17/21） and 45.6%（36/79）, 65.2%（15/23）, 76.1%（16/21） and 82.3%（65/79）, 39.1%（9/23）, 28.6%（6/21）,and distinctly different in statistics, P〈0.01. CONCLUSION： Bcl-2 has a nagetive inducing effect on the cell apoptosis of gastric mucous, which may be resisted and regulated by Bax, p16 could directly inhibit cell proliferation, and mutant p53 is concerned with the development of gastric carsinoma by anti-apoptosis.
出处
《中华肿瘤防治杂志》
CAS
2008年第16期1212-1214,共3页
Chinese Journal of Cancer Prevention and Treatment
关键词
胃肿瘤
癌前状态
癌基因
免疫组织化学
stomach neoplasms, precancerous conditions, oncogenes, immunohistochemistry
