摘要
利用MTT法检测细叶卷柏乙酸乙酯和正丁醇提取物对HeLa细胞生长的抑制作用,利用流式细胞术(FCM)比较不同提取物对细胞凋亡的影响。结果显示:细叶卷柏的乙酸乙酯和正丁醇部位抑制细胞生长和诱导细胞凋亡作用均有明显的剂量依赖性。乙酸乙酯部位的IC50值为1.927μg/mL,正丁醇部位的IC50值为24.600μg/mL。因此,细叶卷柏乙酸乙酯部位的体外抗肿瘤活性相对较强,其次是其正丁醇部位,水提部位相对较弱。细叶卷柏是一种潜在的抗肿瘤药用植物。
MTT assays were used to evaluate effects of ethyl acetate and n-butanol extracts from Selaginella labordei on inhibiting cell proliferation. Flow cytometry （FCM） was used to detect cell apoptosis rate. The results showed that ethyl acetate and n-butanol extracts had a distinctive dose-dependent relation in inhibiting cell proliferation and inducing cell apoptosis. The IC50 values of ethyl acetate and n-butanol extracts were 1. 927μg/mL and 24. 600μg/mL respectively. So ethyl acetate extracts possessed stronger anti-tumor activity,and n-butanol extracts the next,water extracts were relatively weak. S. laborei is a potential medical plant with anti-tumor activities.
出处
《广西植物》
CAS
CSCD
北大核心
2008年第5期690-693,共4页
Guihaia
基金
国家自然科学基金(30470193)~~
