摘要
目的探讨孕期人乳头瘤病毒(HPV)感染及母婴传播情况。方法运用PCR结合DNA探针杂交检测法对2006年1月至12月南京市鼓楼医院不同孕期的405例孕妇宫颈脱落细胞进行HPV-DNA分型检测,阳性孕妇的新生儿出生洗净后对其口咽部和生殖器黏膜细胞进行HPV-DNA分型检测,对各自感染率及感染分型进行分析。结果405例孕妇中71例感染HPV病毒,感染率为17.5%;宫颈光滑组定为宫颈正常组,其HPV病毒感染率为5.6%(12/214);宫颈病变组感染率为30.9%(59/191);孕期被检出的型别有16、18、31、33、35、43、56、58、6、11型,分别占HPV感染的37.7%、7.8%、7.8%、6.5%、1.3%、3.9%、2.5%、10.4%、9.1%、13.0%。57例新生儿中,16例感染HPV病毒,感染率28.1%;感染型别有16、11、6、35、31、58,除1例外均与其母亲感染型别相同,经阴道顺产与经剖宫产分娩的新生儿HPV病毒感染率分别为19.4%(6/31)和38.5%(10/26)。结论孕期存在HPV病毒感染,以16型病毒感染为主,宫颈病变与HPV病毒感染关系密切;HPV病毒存在垂直传播,以16型病毒为主。
Objective To investigate the matenal-fetal vertical transmission of human papilloma virus （HPV） infection of gestational periods, and analyse the positive rate and type of HPV during pregnancy and delivery. Methods Polymerase chain reaction（PCR） assay was employed to detect HPV-DNA in 405 pregnant women. Samples from cervical vaginal exfoliated cells, nasopharyngeal and genital secretion of the newborns were examined after bath . Analyse the infective rate and infective type respectively. Results The study showed that HPV-DNA was detected in the cervical vaginal exfoliated cells of 71 cases（71/405 ）, the detected types of HPV werel6,18 ,31,33 ,35 ,43 ,56,58 ,6 and 11, and the infective rates of each type were 37.7% ,7.8% ,7. 8% ,6. 5% ,1.3% ,3.9% ,2. 5% ,10. 4% ,9. 1% and 13.0% ; HPV -DNA was examined in the nasopharyngeal or genital secretion of the newborns of 16 cases（16/57）. The detected types of HPV-DNA were 16,11,6,35,31,58. The HPV-DNA type of the newborn was the same as its mother except that only one neonate wasn＇t. The infective rates of neonate by normal labor and uterine-incision delivery were 19.4% and 38.5%. Conclusion HPV 16 is the main infection during the trimester of pregnancy. Pregnant women who have the disease of cervix are subjected to the HPV infection. HPV 16 is the main type of mother to child transmission.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2008年第11期839-842,共4页
Chinese Journal of Practical Gynecology and Obstetrics
基金
南京市卫生局科研课题(YKK05091)
关键词
人乳头瘤病毒
垂直传播
妊娠
human papilloma virus
vertical transmission, pregnancy
