摘要
苏云金芽胞杆菌在其整个生命周期中可产生2种不同类型的专一性极强的杀虫蛋白,一种是在其产孢过程中形成的杀虫晶体蛋白(ICPs),包括晶体毒素(Cry)和细胞裂解毒素(Cyt),前者对鳞翅目、鞘翅目、双翅目、鳞翅目和双翅目或鳞翅目和鞘翅目以及线虫具有专一的杀虫活性,后者在体外具广泛的溶细胞活性,但其杀虫谱较窄,杀虫活性较低;另一种是在营养生长期合成并分泌到胞外的非晶体杀虫蛋白即营养期杀虫蛋白(VIPs),具有与杀虫晶体蛋白相似的毒性活性和专一性。然而,这3种蛋白的分子结构和毒性机理各不相同。本文综述了ICP蛋白和VIP蛋白的杀虫机理和害虫抗性产生的分子基础,以及营养期杀虫蛋白的发现和应用对延缓或防止害虫针对ICP蛋白产生抗性的意义。
Bacillus thuringiensis （Bt） produces two kinds of highly specific insecticidal proteins in their life cycle. One is insecticidal crystalline proteins （ICPs） synthesized during sporulation, including crystalline toxin（Cry） and cytolytic toxin （Cyt）. The former is specifically toxic to Lepidoptera, Coleopteran, Diptera, Lepidoptera and Diptera, or Lepidoptera and Coleopteran as well as nematode, while the latter has widely ctolytic activity in vitro, but exhibits narrow insecticidal spectrum and weak toxic activity. Another one is vegetative insecticidal proteins （VIPs） synthesized during vegetative growth period and secreted to outer of cell with similar toxic activity and specificity to ICPs. However, the toxic mechanisms and molecular structures of these three proteins are different from each other. This paper systematically reviewed molecular basis of toxic activity and specificity of ICPs and VIPs and resistance developed by insects, as well as the importance of utilization of VIPs to prevent insect pests from evolving resistance against ICPs.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2008年第5期680-690,共11页
Journal of Huazhong Agricultural University
基金
国家转基因植物研究和产业化专项(2IB-B-02-02)资助
关键词
苏云金芽胞杆菌
杀虫晶体蛋白
营养期杀虫蛋白
毒性活性
害虫抗性
Bacillus thuringinensis
insecticidal crystalline protein
vegetative insecticidal protein
toxic activity
insect resistance
