摘要
目的建立毛细管气相色谱法测定连翘油中a-蒎烯的含量方法。方法采用GC-MS鉴定连翘油中的化学成分,样品经乙酸乙酯稀释,采用分流法进样,以DB-1毛细管色谱柱(30m×0.32mm×0.25μm)为分析柱进行分离测定。结果气质联用法共确定了12种化学成分,α-蒎烯含量测定的平均回收率为100.1%(RSD=0.8%,n=6),重复性的RSD为0.9%(n=6)。结论本方法方便准确,可用于连翘油中α-蒎烯的含量测定。
OBJECTIVE To establish a capillary GC method for the determination of α-pinene in Fructus Forsythiae Oil extracted by SFE-CO2. METHODS The chemical constituents in oil were characterized by GC-MS, the solution was diluted by ethyl acetate, injected into GC by split injection model, and then separated in DB-1 capillary column （30 m×0.32 mm ×0.25 μm）. RESULTS Twelve chemical constituents were identified by GC-MS. The average recovery of α-pinene was100.1% （ RSD was 0.8% ,n =6） , the repeatability RSD was 0.9% （n = 6）. CONCLUSION The method is simple, accurate, can be used to control the quality of Fruetus Forsythiae Oil.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第5期444-446,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
连翘
Α-蒎烯
气相色谱
含量测定
fructus forsythiae
α-pinene
gas chromatography
assay
