摘要
目的:构建可表达HBV X基因的重组腺病毒,观察HBV X基因对肝细胞生物学行为的影响。方法:构建可表达HBV X基因的重组腺病毒Ad-X及空病毒Ad0;重组腺病毒感染HepG2细胞,应用MTT、平板克隆形成试验、流式细胞术、TUNEL等方法观察HBV X基因对肝细胞生物学行为的影响。结果:与HepG2组和Ad0组相比,Ad-X组细胞生长速度较快,其克隆形成率高于对照组,流式细胞分析显示Ad-X感染可加快HepG2细胞G1→S期细胞周期进程;Ad-X组细胞凋亡指数低于HepG2组及Ad0组,Ad-X感染HepG2细胞后可诱导c-myc mRNA的表达。结论:Ad-X可促进HepG2细胞的增殖并抑制其凋亡。
Objective： To investigate the biological impact of the hepatitis B virus gene on hepatoma cells. Methods： Ad - x, the recombinant adenoviruses expressing HBV X gene, was constructed . Hepatoma cells were infected with recombinant adenoviruses. MTT, colony - forming experiment , FCM, TUNEL assay were performed to observe the biological impact of the HBV X gene on liver cells. Results： MTT showed that the Ad - X group cells grew faster than the other group cells. The colony - forming experiment showed that the colonyforming rate of Ad - x group cells was higher thanthat of other group cells. FCM analysis showed that Ad - x infection enhanced the progression of G1→S phase in HepG cell cycle. The apotosis index of Ad - x group cells was lower than that of AdO and control group cells, Semi - quantitative RT - PCR showed the expression level of c - myc was the highest in Ad - x infected cells. Conclusion： Ad - X could facilitate the proliferation activity of HepG2 cells and inhibit its apoptosis in vitro.
出处
《内蒙古医学杂志》
2008年第9期1160-1163,共4页
Inner Mongolia Medical Journal
