摘要
本文详细研究了用乙二胺（en）-铜（Cu2＋）络合物（简作EDAC）测定还原糖的分光光度法．结果表明：Cen／CCu2＋≥2．5即可形成稳定的络合物EDAC，其最大吸光度λmax＝547nm；在介质中吸光度稳定，λmax＝550nm；于NaOH介质中吸光度下降，波长略为红移．在固定介质，或中，EDAC性质一年同内未发现变化，能与还原精作用生成Cu2O沉淀，而本身的量减少，其吸光度与还原糖在0．063～1．64mg（六碳糖）内线性相关，相关系数绝对值｜ρ｜＞0．998．实测样品回收率在98％～103％之间．本法试剂易得、性稳定，操作简便，取样量少．灵敏度较高．en是一种常用试剂，有关用EDAC络合物测定还原糖的方法尚未见报导．
Spectrophotometry for the determination of reducing sugar using ethylenediamine-cupric ion complex(EDAC)has been studied in detail. EDAC had a stable maximum absorbance at the wavelength (λmax)of547nm in the ratio of Cen to CCu2+ equal to or more than 2. 5. In the medium,or, its maximum absorlance was stable too and its λmax was 550nm. In the medium,NaOH,there was a decrease in its absorbance and a slight red-shift in λmax. In or,as a certainmedium,no change in its properties was found within one year. It could react with reducing sugar to producecuprous oxide,Cu2O(red deposition),while the concentration of EDAC was being decreased. There was a linear dependence between the absorbance of EDAC and the quantity of reducing sugar in the range 0. 063mg～1. 64mg (hexose). The correlation coefficient (|ρ|)is more than 0. 998. The recovery percents of measuredsamples were beween 98%and 103%. This method has a simple procedure with less sampling quantity and ahigher sensitivity. The reagents in it are easy to get and stable in their properties. en is a common reagent.The method about using EDAC in the determination of reducing sugar has not yet been found in the press.
出处
《新疆大学学报（自然科学版）》
CAS
1997年第2期69-73,共5页
Journal of Xinjiang University(Natural Science Edition)
