摘要
目的研究甘油二酯的减肥效果及可能机制。方法(1)5wSD大鼠随机分为3组,每组10只,对照组[饲料甘油三酯(TG)含量7%],高甘油三酯组(TG20%),高甘油二酯(DG)组(DG20%)自由进食喂养8w。实验期间记录动物进食量、排泄量、体重变化,分析脂肪消化率。实验结束后检测动物腹部脂肪重量、血清生化指标变化。(2)餐后血浆脂质动态分析实验采用13w雄性Wistar大鼠(n=50),分两组分别灌胃10%DG或10%TG乳化物,灌胃量0.73ml/100gbw。不同时间麻醉,颈静脉、门静脉分别取血,测血浆中游离脂肪酸(FFA)和TG含量(每次5只)。(3)酮体实验采用6w雄性Wistar大鼠,随机均分三组,分别用20%DG和20%TG乳化物灌胃,对照组用等能量葡萄糖水溶液(GS)作安慰剂。代谢笼中饲养6d,灌胃量0.5ml/100gbw,连续收集144h的尿液,测尿中总酮体量。结果DG组腹部脂肪重量、体重增加及血清TG水平均明显低于高TG组。DG组大鼠门静脉FFA水平明显高于TG组,而颈静脉TG水平略低于TG组,而尿酮体量显著高于TG组。结论膳食DG能明显抑制实验大鼠体重增加,降低血清中性TG水平,与TG组相比,其减肥降血脂效果可能源于其代谢途径不同。
Objective To study the effects of diacylglycerol （DG） on body weight of rats and its possible metabolic mechanism. Method （1） SD rats （n=30） were randomly divided into 3 groups. They were given free access to diets containing 7% （wt） triacylglycerol （TG, control group）, 20% （wt） TG or 20% （wt） DG diets, respectively for 8 w. The changes of body weight, height, food intake, and feces were recorded. At the end of experiment, abdominal fat weight （including perirenal fat and epididymal fat）, blood lipids were detected. （2） 13 w male Wistar rats （n=50） were divided into 2 groups （administrated with 10% TG or DG emulsion, respectively） in postprandial blood fat profile experiment. Blood lipids of 5 rats were analyzed at desired interval. （3） 6 w male Wistar rats （n=30） were randomly divided into 3 group, control group, DG group and TG group, administrated with glucose solution, 20%DG emulsion and 20%TG emulsion respectively for 6 d. Urine in 144 hr was collected continuously and analyzed for total ketone bodies. Results High DG diet resulted in a significant reduction in both body weight gain, ratio of abdominal fat to body weight and serum TG levels compared with the high TG diet. DG group have higher FFA level in portal vein and lower TG level in jugular vein than those of high TG group. But urine ketone body level of high DG group was higher than high TG group. Conclusion Dietary DG reduced fat accumulation in abdominal region, body and blood, and these effects may be involved with different metabolic ways of DG compared with TG.
出处
《营养学报》
CAS
CSCD
北大核心
2008年第5期443-446,共4页
Acta Nutrimenta Sinica
基金
浙江省科技厅面上项目(No.2007c32024)
关键词
甘油二酯
减肥
餐后血脂
酮体
diacylglycerol
antiobesity
postprandial blood fat
ketone body
