摘要
利用表面嫁接法制备了氰基（-CN）、乙烯基（-CH=CH2）、氨基（-NH2）和巯基（-SH）等有机基团功能化介孔材料R-MCF，并用作制备固定化青霉素酰化酶（PGA）的载体。对比研究了R-MCF对PGA的负载速率和固定化酶PGA／R—MCF对水解青霉素G钾制备6-氨基青霉烷酸的催化活性。结果表明，疏水性载体NC—MCF、CH2=CH—MCF和HS—MCF对PGA的负载速率高于具有亲水性的H2N—MCF和MCF；固定化酶PGA／NC-MCF和PGA／CH2-CH—MCF的表观活性分别为1790IU／g和1667IU／g，明显高于PGA／MCF。
Organic functionalized mesoporous material R-MCF with vinyl （-CH = CH2 ）, cyano （--CN）, amino （--NH2） and thiol （--SH） groups were prepared by post-synthesis graft method and used as the supports for immobilizing penicillin G acylase （PGA）. The PGA loading rate on the R-MCF with different organic groups and the apparent activity of the immobilized PGA for hydrolysis of penicillin G potassium to produce 6-aminopenicillanic acid were comparatively studied. The results indicated that the PGA loading rate on the hydrophobic CH2 = CH-MCF, NC-MCF and HS-MCF were higher than that on the hydrophilic H2N-MCF and MCF. The apparent activity of PGA/NC-MCF and PGA/CH2 = CH-MCF are 1790 IU/g and 1667 IU/g, respectively, which are obviously higher than that of PGA/MCF.
出处
《石油学报（石油加工）》
EI
CAS
CSCD
北大核心
2008年第B10期175-178,184,共5页
Acta Petrolei Sinica(Petroleum Processing Section)
基金
国家自然科学基金项目（50662004）资助
关键词
介孔材料
功能化
青霉素酰化酶
固定化
mesoporous material
functionalization
penicillin G acylase
immobilization
