摘要
目的分析血液分析仪XE-2100血小板计数的准确性和血涂片复检的关系。方法测定血液分析仪XE-2100、CD-3700和手工法血小板计数精密度、线性和相关性;选择仪器CD-3700随机检测有报警的标本用XE-2100和手工法计数血小板;比较仪器血小板计数在不同范围内的标本例数与标本血片血小板数量估计值例数的符合率。结合观察血涂片红细胞、白细胞形态,与血液分析仪血小板计数报警进行对照比较。结果XE-2100血小板计数精密度较高,变异系数<3%,血小板计数有良好的线性。其PLT-O法和PLT-I法血小板计数与手工法血小板计数相关性为γ=0.9。标本血小板浓度越低,仪器血小板计数与血片血小板估计值符合率越低。XE-2100血小板计数报警"血小板凝集"、"PLT分布异常"分别与血片上"大血小板"、"红细胞碎片"、"小红细胞增多"的例数相关。血小板计数在正常范围(100～300)×109/L时,仪器PLT-O法和PLT-I法血小板计数的报警与血片上所见血小板形态最为一致。结论XE-2100血小板计数准确性较高;但当血小板浓度异常、特别是浓度减低时,仪器计数结果需结合散点图、报警内容进行血片显微镜复查。
Objective To analyze the relationship of platelet count accuracy between hematology analyzer XE-2100 and blood smear reviews. Methods The precision,linearity and correlation of platelet count were determined by hematology analyzer XE-2100,CD-3700 and manual method respectively; The samples with platelet count flags out of CD-3700 were selected to be measured again by both XE-2100 and manual method; the agreement rates of platelet count by hematology analyzers with the different concentration were compared separately those under microscopy. The morphology of red blood cells and white blood cells on blood smears were also referred when the flags showed on the analyzers. Results On hematology analyzer XE-2100, the precision of platelet count showed high with the coefficient variation of 2% and fair linearity. The linear correlation of platelet count was γ=0.9 between the methods by PLT-O or PLT-I on XE-2100 and method by microscope. The more the platelet concentration reduced,the more the agreement of platelet count decreased between XE-2100 and blood smears. The flags “platelet clumps” or “abnormal platelet distribution” indicated on XE-2100 were related to the increased “large platelet”, “red blood fragment” and “small red blood cell” on blood smears. The agreement of platelet count flags between the methods by PLT-O and PLT-I on XE-2100 reached the highest when the platelet concentrations were normal range. Conclusion The hematology analyzer XE-2100 showed high accuracy platelet count. Microscope reviewing is necessary when the abnormal scatergrams and flags displayed on XE-2100 or abnormal platelet concentration.
出处
《实验与检验医学》
CAS
2008年第5期484-488,共5页
Experimental and Laboratory Medicine
关键词
血液分析仪
血小板计数
血涂片
复检
Hematology analyzer
Platelet count
Blood smear
Review
