摘要
目的用正交试验法优选老鹳草中总黄酮的提取工艺。方法以总黄酮提取量为评价指标,在确定醇浓度的基础上,选择乙醇用量(倍)、提取时间(h)、提取温度为考察因素,采用正交试验法L9(34)确定醇提取老鹳草中总黄酮的最佳工艺。结果最佳提取工艺条件为60%乙醇20倍量提取75min,提取温度为显著影响因素。结论可为老鹳草药效学的进一步研究和资源开发利用提供科学依据。
Objective To study the optimum extraction process of total flavonoids in Geranium wilfordii Maxim Methods The extraction of total flavonoids was choosen as the assessment index, temperature, ratio of solid-liquid and extracting time were the studing factors the optimum ethanol extraction process was selected with the orthogonal design. Results The optimum ethanol extracting process conditions were as follows： adding 20 fold of 60% ethanol, extracting for 75 minutes, the temperature was 80℃ and extracting time is predominant in- fluencing factor. Conclusion To provide a scientific basis for Geranium wilfordii maxim pharmacodynamics of further research and development and utilization of resources.
出处
《中国现代药物应用》
2008年第21期10-11,共2页
Chinese Journal of Modern Drug Application
关键词
老鹳草
总黄酮
正交实验
Geranium wilfordii
Total flavonoids
Orthogonal design
