摘要
目的：探讨血清ＨＢＶＤＮＡ浓度与ＨＢＶ免疫学标志的关系。方法：应用竞争性ＰＣＲ方法，检测ＨＢＶ免疫标志不同背景的原发性肝癌（ｎ＝３８），肝硬化（ｎ＝３６），慢性肝炎（ｎ＝５１）共１２５例的血清ＨＢＶＤＮＡ浓度。结果：ＨＢＶＤＮＡ浓度与血清ＨＢＶ免疫标志有关，而与慢性肝病类型无关；五项免疫标志均阴性或抗－ＨＢｓ阳性的慢性肝病患者中，约有三分之一病例存在低水平ＨＢＶ感染；ＨＢｅＡｇ阳性病例的ＨＢＶＤＮＡ浓度总体上明显高于抗ＨＢｅ阳性组，但后者中部分病例的ＨＢＶＤＮＡ浓度也较高。结论：血清ＨＢＶＤＮＡ浓度与ＨＢＶ免疫标志有一定的对应关系，但存在个体差异。
Objective: To investigate the relationship between serum HBV DNA concentration and HBV immunological markers. Methods: Serum HBV DNA concentration was examined with competitive polymerase chain reaction in 38 cases of primary hepatic carcinoma, 36 liver cirrhosis and 51 chronic hepatitis with different backgrounds for serum HBV immunological markers. Results: Serum HBV DNA concentration was correlated with HBV immunological markers but not with the type of chronic liver diseases and the level of serum alanine aminotransferase. About one third of the cases negative to all five HBV immunological markers or positive to anti HBs had low level of viral replication. In general, the serum HBV DNA concentration was significantly higher in the cases positive to HBeAg than in those positive to HBe. But high concentration was also found in some of the cases positive to HBe. Conclusion: Serum HBV DNA concentration is correlated to HBV immunological markers but there still exists difference among individuals.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第6期527-529,共3页
Journal of Third Military Medical University
关键词
竞争性
聚合酶链反应
乙型肝炎病毒
免疫标志
competitive polymerase chain reaction
hepatitis B virus
chronic liver disease
