摘要
目的：观察人肺癌裸鼠移植瘤整合蛋白表达与其转移的相关性。方法：以ＡＢＣ免疫组化染色，对转移力由低到高依次为ＰＬＡ－８０１Ｃ、Ａｎｉｐ９７３、ＰＬＡ－８０１Ｄ、ＰＬＡ－８０１ＤＬ四株人肺癌裸鼠移植瘤作整合蛋白亚单位表达的观察，并在裸鼠体内观察整合蛋白β１抗体及其配体ＲＧＤ多肽对移植瘤生长及转移的影响。结果：四株移植瘤均呈α２、α５、α６Ｂ阴性。β１的表达除Ａｎｉｐ９７３呈强阳性、ＰＬＡ－８０１ＤＬ呈弱阳性外，也均呈阴性。但四株移植瘤除ＰＬＡ－８０１Ｃ外，其余３株均呈α６Ａ强阳性。β１抗体及ＲＧＤ多肽对ＰＬＡ－８０１ＤＬ移植瘤的转移不见明显影响。结论：４株人肺癌移植瘤总体上呈β１亚族整合蛋白低表达，表达水平与其转移无显著相关。
Objective: To investigate the relationship between the expression of integrin of transplanted human lung carcinoma and its metastasis in nude mice. Methods: The expression of integrin subunits were observed with ABC immunohistochemistry in four strains of xenografts of human lung carcinoma. The metastatic potential of the four xenografts was from low to high among PLA801C, Anip973, PLA801D and PLA801DL in nude mice. The effects of β1 antibody and RGD peptide on the PLA801DL were also studied. Results: α2,α5 and α6 B were all negatively expressed. The expression of integrin subunit β1 was only strongly positive in Anip973 and weakly positive in PLA801DL. But high expression of α6 B was found in PLA801D, PLA801DL and Anip973 except PLA801C. β1 subunit antibody and RGD peptide exerted no marked effects on metastasis of PLA801DL xenograft. Conclusion: Low expression of integrin β1 subfamily exists in four strains of xenografts of human lung carcinoma but is not correlated to their metastatic capacity.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第6期500-502,共3页
Journal of Third Military Medical University
基金
重庆市中青年科技专家基金
关键词
整合蛋白
肺肿瘤
肿瘤转移
裸鼠
integrin
human lung carcinoma
metastasis
nude mouse
