摘要
为探明大豆GmAGL15基因的表达调控规律,应用电子克隆技术从大豆基因组中克隆了GmAGL15 1000 bp的启动子序列,用PLACE在线启动子预测工具分析表明:该序列含有启动子的一般结构,如TATA-BOX、CAAT-BOX。另外还含有一些顺式作用元件,如调控GmAGL15的组织特异的和特定发育阶段的表达,对胁迫的应答,对光的响应,以及反馈调节,推测大豆GmAGL15基因表达有相应组织特异性,可能受蔗糖、生长素和乙烯等的调控。用FootPrinter和PLACE在线工具对大豆与拟南芥等其他4种植物的同源基因启动子的顺式元件进行比较,发现不同植物的启动子既有保守性,又有多样性,转录因子结合位点的分布相似,但也有区别,暗示了GmAGL15基因表达调控的精确性或多样性。
In order to study GmAGL15 gene expression and regulation in soybean,its1000 bp promoter sequence was cloned from the soybean genome in silico. Promoter sequence analyzed by PLACE showed that it had TATA-box, CAAT-box and some cis-acting elements which could regulate specific expression in different organs and response to stresses, light, and self-feedback. In addition, GmAGL15 may be regulated by sucrose, auxin and ethylene. Comparaison of the promoter of GmAGL15 with homologues from other plants by FootPrinter and PLACE suggested that these promoters had conserved and diverse domains, while the distribution of transcription factor-binding sites had similarity and difference. These findings implied the accuracy and diversity of GmAGL15 gene expression and regulation.
出处
《大豆科学》
CAS
CSCD
北大核心
2008年第5期727-731,共5页
Soybean Science
基金
国家自然科学基金资助项目(30771362)
国家高技术研究发展计划(863计划)资助项目(2006AA10Z1C1)
教育部高等学校引智计划(111计划)资助项目(B08025)
关键词
大豆
GmAGL15
启动子
电子克隆
序列分析
Soybean （ Glycine max L, Merr）
GmAGL15
Promoter
In silico cloning
Sequence analysis
