摘要
为了研究食管癌前病变组织中多种抑癌基因表达状况,我们应用P_(53)、P_(16)和nm23蛋白对74例食管癌旁异型增生组织进行了检测。结果:P_(53)和P_(16)蛋白均随异型增生病理级别的升高而标记阳性率升高,Ⅰ级异型增生P_(16)阳性率与Ⅱ～Ⅲ级比较有显著差异(P<0.005);nm23蛋白随异型增生病理级别的升高而标记阳性率下降,Ⅰ～Ⅱ级阳性率与Ⅲ级比较差异有显著性(P<0.005);P_(53)+P_(16)+nm23阳性者8例(10.8％),P_(53)+P_(16),P_(16)+nm23,P_(53)+nm23阳性者25例(33.8％),P_(53),P_(16)和nm23阳性者34例(45.9％),1种以上抑癌基因蛋白阳性者67例(90.5％)。结果提示:食管癌前病变存在P_(53)和P_(16)蛋白过度表达,P_(53)和P_(16)基因突变是食管癌发生的早期事件;nm23蛋白表达水平与异型增生病理级别呈负相关,说明nm23基因不仅能抑制肿瘤转移,而且能部分地抑制肿瘤形成;食管癌前病变存在多种抑癌基因突变或失活。
For study on the expressions of multiple tumor suppressor genes protein of primalignant esophageal lesions, seventy-four cases
of squamous cell dysplasias near the esophageal squamous cell carcinomas were invastigated by P53, P16 and nm23 protein. It was
found that the positive rates of P53 and P16 protein increased with the pathological grades of dysplasia. the positive rate of P16
protein was significantly higher in dysplasia with grade II ~ III than that in dysplasias with grade I (P<0. 005); however, the
positive rate of nm23 protein decreased with the pathological grades of dysplasia, it was significantly higher in dysplasia with grade
I^II than that in dysplasia with grade III (P<0. 005) ;Eight of the 74 dysplasias (10.8%) showed P53+Pl6+nm23 three tumor suppressor gene proteins co-expression, twenty-five (33. 8%) showed P53+P16. P16+nm23 and P53+nm23 two tumor sup
pressor gene proteins co-expression, thirty-four (45. 9%) showed only one tumor suppressor gene protein expression. The results
indicated that P53 and P16 expressions were excessive in premalignant esophageal lesions and suggested, that the P53 and P16 ex
pressions may be an early event in development of the squamous carcinoma of the esophagus; in addition, there was an inverse
relationship between the expression of nm23 and the pathological grade of the dysplasia, and suggested that the nm23 gene could
not only inhibit the tumor formation in part; there may be multible tumor suppressor genes mutation or lost in premalignant
esophageal lesions. To study on expressions of multiple tumor sappressor genes in premalignant esophageal lesion is significant for
provention and treatment of esophageal carcinoma.
出处
《齐齐哈尔医学院学报》
1997年第4期241-243,共3页
Journal of Qiqihar Medical University
