摘要
研究了３种有机锗化合物：β－羧乙基锗倍半氧化物、β－（α－甲基）羧乙基锗倍半氧化物、二－（β－羧乙基）锗氢氧化物的离子色谱法分离和测定条件。以四硼酸钠溶液为淋洗液，硫酸溶液为再生液，梯度淋洗。３种化合物均达到基线分离，检出限分别为０．０３８，０．０３５和０．０２５ｍｇ／Ｌ（以锗计）。方法已用于保健口服液的测定，加标回收率为９５％～１０１％，其结果与氢化物发生－原子荧光光谱法的结果吻合。
A new high performance ion exchange chromatographic method for separation and determination of three organogermanium compounds β carboxyethylgermanium sesquioxide(Ⅰ), β (α methyl)carboxyethylgermanium sesquioxide(Ⅱ) and di (β carboxyethyl)germanium hydroxide(Ⅲ) has been developed. A Dionex DX 300 Ion Chromatograph equipped with a Dionex PED Ⅱ pulsed electrochemical detector (conductivity mode), a Dionex AMMS 1 anion micromembrane suppressor, and a Dionex ACI advanced computer interface coupled with AI 450 chromatographic software was employed. The separation was achieved by using a Dionex IonPac AS4A SC column as analytical column, sodium tetraborate solution as eluent, and sulfuric acid solution as regenerant. For reducing run time, a gradient program was chosen. The detection limits(S/N=3, expressed as germanium) for the three compounds were 0.038mg/L(Ⅰ), 0.035 mg/L(Ⅱ) and 0.025 mg/L(Ⅲ), respectively. The method has been applied to the analysis of two tonic oral drinks, and the average recoveries for the three compounds ranged from 95% 101%. The results obtained were in agreement with those of hydride generation atomic fluorescence spectrometry (HG AFS).
出处
《色谱》
CAS
CSCD
北大核心
1997年第3期240-242,共3页
Chinese Journal of Chromatography
关键词
离子色谱法
有机锗化合物
保健口服液
ion chromatography, organogermanium compound, tonic oral drinks
