摘要
在水产养殖领域倍受重视的药物动力学的应用可有效地避免虾病用药的盲目性。氯霉素被认为是抑制虾原弧菌作用最强的药物，实验中取平均体长９．５ｃｍ的样本虾，经暂养后一次性投喂定量药饵，然后在不同时间间隔取样本虾速冻保存，经适当预处理后测定各组织内氯霉素含量。应用高效液相色谱法进行了药物动力学研究［１］，分离条件：５μｍＣ１８柱，ＭｅＯＨ－Ｈ２Ｏ（３０７０，Ｖ／Ｖ）洗脱液，Ｍ４９０型紫外检测器。方法快速、准确，在１～５０ｍｇ／Ｌ浓度范围内线性关系良好，相关系数为０．９９９５。
The pharmacokinetic in the aquatic was valued recently. It is advantageous to avoid blind with medicine in breed penaeus chinensis. In this paper, studies on the pharmacokinetic paremater of chloromycetin in penaeus chinensis. Chloromycetin is the stronger medicine in controling penaeus disease. The 2‰ contents of antibiotic chloromycetin was added into penaeus chinensis feed producted with conventional method. The length of penaeus sample was 9.5cm. The test makes the penaeus chinensis be feeded medicine only one time and get samples of penaeus in different times then cooled immediately. Before analysis dissect, weigh, pre treat properly and then determinate contents in muscle, blood, intestinesstomach and hepatopancreas. The method for pharmacokinetic study on antibiltic chloromycetin in penaeus chinensis body was established by HPLC determinaton. The operating conditions were μBondapakC18 column, 30∶70(V/V) MeOH/H 2O eluent and UV detector. The method is more rapid, accurate and sensitive. The correlation coefficient of the calibration curve is 0.999 5 in the range of 150mg/L.
出处
《色谱》
CAS
CSCD
北大核心
1997年第5期431-434,共4页
Chinese Journal of Chromatography
基金
山东省科委基金
关键词
高效液相色谱
药物动力学
氯霉素
对虾
虾病防治
high performance liquid chromatography, pharmacokinetic, chloromycetin, penaeus chinensis
