摘要
为明确龈沟液中有无影响白细胞介素８（ＩＬ８）功能的因子及其活性如何，作者将ＩＬ８１μｇ／Ｌ加入龈沟液样本中（成人牙周炎７例，牙周健康对照８例），用夹心ＥＬＩＳＡ法检测各样本中ＩＬ８的含量。结果：成人牙周炎组（ＡＰ组）的ＩＬ８含量显著低于健康对照组（Ｐ＝０．００１），其检出值分别为００５μｇ／Ｌ±０．０３μｇ／Ｌ和０３６μｇ／Ｌ±０１５μｇ／Ｌ。单纯ＧＣＦ组趋化白细胞的总数高于生理盐水对照组。当在ＧＣＦ中加入ＩＬ８，从０１μｇ增至１μｇ时，小鼠趋化的白细胞总数增长率为１８６％，而含同样剂量ＩＬ８的生理盐水对照组的增长率为４９１％。在从含００１μｇ４μｇＩＬ８的各对照组，白细胞趋化反应曲线呈倒“Ｖ”形。ＩＬ８为１μｇ时，趋化的白细胞数达到高峰。结论：ＧＣＦ中存在ＩＬ８的抑制因子，成人牙周炎ＧＣＦ中，此抑制因子的含量显著升高；成人牙周炎ＧＣＦ具有较强的白细胞趋化活性；但ＧＣＦ中的ＩＬ８抑制因子，对由ＩＬ８诱导的白细胞趋化反应有一定的抑制活性；ＩＬ８对白细胞的趋化反应曲线呈倒“Ｖ”形。因此，评价和研究ＩＬ８的功能及疾病状态下的ＩＬ８所具有的意义，应该至少分高、低两个剂量区别?
In order to comfirm the Interleukin8 (IL8) existence of inhibitor and its biological activity in gingival crevicular fluid (GCF), this study examined the GCF taken from 7 adult periodontitis (AP) patients. In neutralization test of IL8, the results showed that the mean level of IL8 was less than 1ng/ml, which had been added into the GCF before ELESAs was performed to measure the amount of IL8 in GCF. The mean level of IL8 in the GCF of AP group was significantly lower than that of healthy group (P<0.001). In biological activity test of IL8 inhibitor, using pooled GCF taken from 8 AP patients (23 teeth), the results showed that the GCF(without recombinant human IL8, rhIL8) caused more white blood cell (WBC) migration than blank control group (physiological saline) did. When the amount of rhIL8 increased in GCF from 0.1 μg to 1 μg, the WBC count increased by 18.6% which was less than the increase rate (49.1%) in control group with same dose of IL8. In the saline group containing rhIL8, the WBC chemotactic response appeared as an inverted “V”shape curve. All these data suggested that ①Certain kinds of IL8 inhibitor exist in GCF which can “cleave” IL8. ②The level of IL8 inhibitor(s) increases significantly in the GCF from periodontitis sites. ③ The GCF of adult periodontitis patient has strong chemotactic effects on WBC. IL8 inhibitor(s) in GCF can slightly suppress the chemotactic effect induced by IL8. When assessing the role of IL8 in pathophsiology, the high and low dose of IL8 might have different sense.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1997年第6期375-377,共3页
Chinese Journal of Stomatology
关键词
白细胞介素8
抑制因子
免疫
龈沟液
牙周炎
Interleukin8 Suppressor factor, immunologic Gingival crevicular fluid Periodontitis
