摘要
目的了解四川省甲3亚型流行性感冒（流感）病毒血凝素（Hemagglutinin,HA）的抗原性和基因变异情况,比较四川省2006-2007年度甲3亚型流感病毒流行株与世界卫生组织（WHO）推荐的2006-2007年度北半球甲3亚型流感疫苗株甲/威斯康星/67/2005（H3N2）HA1区的基因差异,为评价流感疫苗免疫效果提供依据。方法采集四川省流感监测哨点医院和疑似流感疫情的流感样病例咽拭子,用狗肾传代细胞进行病毒分离。选取来自不同时间、地点,且经血凝抑制（Hemagglutination Inhibition,HI）试验鉴定为甲3亚型的流感病毒分离株进行单向HI试验;提取病毒核酸,进行逆转录-聚合酶链反应扩增HA基因的HA1区,对扩增产物进行序列测定并推导出氨基酸序列;将分离株的序列与甲/威斯康星/67/2005（H3N2）的HA1区序列进行比较,并进行基因进化特征分析。结果单向HI试验表明,2006-2007年度四川省甲3亚型流感毒的抗原性与中国甲3型流感标准株（甲/云南/1145/2005和甲/江西东湖/312/2006）相比,HI效价均无≥4倍的差异。核苷酸和氨基酸序列分析结果表明,核苷酸和氨基酸序列的同源性分别为99.59%和99.47%,仅有4-6个氨基酸位点发生了替换,涉及2个抗原决定簇。结论四川省2006-2007年度甲3亚型流感病毒的抗原性和基因特性未发生明显变异。
Objective To study the genovariation and antigenicity status about hemagglutinin of influenza virus subtype A3 circulated in Sichuan province;compare the genic differences on HA1 regions between influenza virus subtype A3 circulated in Sichuan province and influenza vaccine strain of subtype A3（A/Winsconsin/67/2005（H3N2））recommended by WHO for northern hemisphere in 2006～2007 so as to provide theoretical basis for evaluating the immunization effect of influenza vaccine.Methods The throat swab from the suspected influenza cases were collected from suspected epidemic influenza areas and the sentinel hospital monitored in Sichuan,and virus were isolated by using MDCK cells.The influenza virus isolated strain collected from different time and locations was identified to be influenza virus subtype A3 by hemagglutination inhibition test,then the isolated strains were performed one-way hemagglutination inhibition test;the viral nucleic acid were extracted to amplify the HA1 regions of HA by using RT-PCR,and the amplified products were sequenced to deduct the amino acid sequence;the sequence of isolated strains was compared with the sequence in HA1 of influenza vaccine strain of subtype A3（A/Winsconsin/67/2005（H3N2））recommended by WHO for northern hemisphere in 2006～2007,followed by signature analysis of the gene evolution.Results In line with results of one-way hemagglutination inhibition test,the variance of hemagglutination inhibition valence showed to be less than 4 times difference when the antigenicity of influenza virus subtype A3 compared with type strain of influenza virus subtype A3 in China（A/Yunnan/1145/2005 and A/Donghu of Jiangxi/312/2006）.The results of nucleotide and amino acid sequence demonstrated that the similarities of nucleotide and amino acid sequence were 99.59% and 99.47% respectively.Just 4 to 6 amino acids sites replaced,which involved 2 antigen determinants.Conclusion There was no significant mutation of antigenicity and genetic characteristics of influenza virus subtype A3 circulated in Sichuan during 2006～2007.
出处
《中国疫苗和免疫》
CAS
2008年第5期437-439,共3页
Chinese Journal of Vaccines and Immunization
基金
四川省卫生厅科研课题(编号070110)
