摘要
通过冷冻干燥方法制备出仿生型多孔结构的溶胶-凝胶生物活性玻璃(BG)/胶原蛋白(COL)/磷酸丝氨酸(PS)支架材料,并评价了小鼠胚胎成骨细胞系细胞在BG、BG-COL、BG-COL-PS支架材料上的生长、黏附、增殖情况。MTT法测结果显示,细胞在材料表面贴附、增殖良好,且细胞在BG-COL-PS支架材料生长增殖要明显好于BG-COL、BG支架材料。BG-COL-PS支架材料有可能成为一种新型的骨组织工程支架材料。
In the present study, novel biomemetic composite scaffolds using the sol-gd derived bioactive glass（BG）, collagen（COL）, and phosphoserine{PS） were prepared by freeze-drying. MC3T3-E1 were cultivated in vitro, collected and seeded onto the surface of 13G, I^-COL and t^-COL-PS. Cell attachment and proliferation were observed. The cell proliferation was tracked by MTT method 1,3,5 d after seeding. MTT showed that the cells can adhere to and proliferate well on the surface of the scaffolds, and the cell proliferation result of scaffold BG-COL-PS was better than those of scaffolds BG and BG-COL. Therefore, the scaffold BG-COL-PS can be a potential scaffold for tissue engineer.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2008年第5期1112-1115,共4页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(50672028
50472054)
广东省自然科学基金资助项目(05006587
04205786)
国家973项目资助(2005CB623902)
广州市科技计划项目资助(2003Z1-D0021)
