摘要
目的探讨地塞米松(dexamethasone,DEX)对人羊膜上皮细胞Wish细胞株水通道蛋白8 (aquaporin 8,AQP8)表达的影响。方法培养人羊膜上皮细胞Wish株,应用1μg/mL,10μg/mL,20μg/ mL,40μg/mL,100μg/mL地塞米松培养液[实验组(1μg/mL组、10μg/mL组、20μg/mL组、40μg/mL组、100μg/mL组)]孵育48 h;对照组为未加地塞米松处理的人羊膜上皮细胞Wish株,采用免疫荧光细胞化学法(immunofluoresecnce chemistry)、RT-PCR法、Western blot法,检测在不同浓度地塞米松作用下,Wish细胞AQP8 mRNA及蛋白表达水平。结果人羊膜上皮细胞Wish株可见。AQP8 mRNA及蛋白表达。10μg/mL,20μg/mL,40μg/mL,100μg/mL地塞米松对人羊膜上皮细胞Wish株AQP8 mRNA及蛋白表达,具有增强性调节作用。其中,40μg/mL组的AQP8 mRNA及蛋白表达水平达峰值,与对照组比较,差异有显著意义(P<0.05)。结论地塞米松可增加羊膜上皮细胞AQP8的表达,从而为治疗羊水异常提供新的思路。
Objective To explore the effect of dexamethasone （DEX） on the expression of aquaporin 8 （AQP8） in human Wish cells. Methods Human Wish cells were cultured, then were treated with Dexamethasone for 48 hours at the concentration of 1 μg/mL, 10 μg/mL, 20 μg/mL, 40 μg/mL, 100 μg/mL（the test group）. Human Wish cells were not treatment with Dexamethasone as the control group. The variation expression of aquaporin 8 mRNA and protein in Wish cells were observed by immunofluoresecnce chemistry, RT-PCR and Western blot. Results The expression of aquaporin 8 mRNA and aquaporin 8 protein in the test group was increased compared with those in the control group. When the concentration of dexamethasone was 40 μg/mL, the expression of aquaporin 8 mRNA and protein was in the peak and had a significant difference compared with that in the control group （P〈 0.05）. Conclusion Dexamethasone could increase the expression of aquaporin 8 mRNA and aquaporin 8 protein in human Wish cells, and provide a new way for treating abnormal capacity of amniotic fluid.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2008年第5期5-7,共3页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
国家自然科学基金资助项目(30400616
30672244)
重庆市人口计生委科研基金(2004-65)~~
