摘要
以苦瓜(Momordica charantia L.)的各外植体为试验材料,研究不同抗生素种类和浓度对苦瓜培养的影响;比较了在添加不同种类和浓度抗生素的MS培养基中,苦瓜不同外植体及对应愈伤组织诱导和增殖。结果表明:卡那霉素是用于筛选转化植株的最佳抗生素,100 mg/L是抗性筛选的最适宜浓度。
The explants of Momordica charantia were elected to study the effects of different antibiotics at various concentrations on their growth.The induction and growth of callus was compared after putting different explants in the MS culture media antibiotics at different concentrations.Variance analysis and overall observation indicated that the most proper antibiotic for selecting transformed explants and callus was Kanamycin,and the best concentration for resistance selection was 100 mg/L.
出处
《北方园艺》
CAS
北大核心
2008年第10期18-21,共4页
Northern Horticulture
基金
教育部博士点基金资助项目(2002061088)
关键词
苦瓜
抗生素
组织培养
Momordica charantia L.
Antibiotic
Tissue culture
