期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

慢病毒载体介导的转基因整合位点研究方法 被引量：3

Research Approaches for Lentiviral Vector mediated Transgene Integration Site
原文传递
导出
摘要 慢病毒载体整合到宿主细胞的染色体上可以长期稳定表达,目前已成为基因治疗和转基因动物载体研究的热点。慢病毒载体整合的位置效应是影响外源基因表达的重要因素,慢病毒载体整合位点的研究是探索外源基因整合机制的手段之一。转基因整合位点研究的方法主要有5种:荧光原位杂交、个体基因组文库筛选法、反向PCR、接头PCR、锚定PCR。近来的研究后发现整合位点之间可能存在一些共同特征。 Lentiviral vector is characterized with its stable integration into host genome and long-term expression in host cells, thus becoming one of the hotspots in development of gene therapeutic strategies and transgenic animal models. Integration position of lentiviral vector is a key factor affecting expression of transgene. To study the mechanism of transgene integration in host genome, first step is to clone the sequence of integration site. The approaches for this purpose include FISH, selection of individual genome library, inverse PCR, adaptor-PCR and anchored PCR. Different methods are conformed to different situations depending on how transgenes are produced. Recent studies have demonstrated that there appear some common characteristics among the integration-site sequences.
作者 孟坡 任兆瑞
机构地区 上海交通大学医学遗传研究所
出处 《医学分子生物学杂志》 CAS CSCD 2008年第5期436-439,共4页 Journal of Medical Molecular Biology
基金 国家重点基础研究发展规划("973"计划)(No.2004CB518806)~~
关键词 慢病毒载体 整合位点 研究方法 lentiviral vector integration site research methods
分类号 Q782 [生物学—分子生物学]
  • 相关文献

参考文献19

  • 1[1]NALDINI L,BLOMER U,GAGE F H,et al.Efficient transfer,integration,and sustained long-term expression of the transgene in adult at brains injected with a lentiviral vector[J].Proc Natl Acad Sci USA,1996,92 (21):11382-11388.
  • 2[2]MIYOSHI H,SMITH K A,MOSIER D E,et al.Transduction of human CD34+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors[J].Science,1999,283 (5402):682-686.
  • 3[3]KAFRI T,BLOMER U,PETERSON D A,et al.Sustained expr-ession of genes delivered directly into liver and muscle by lentiviral vectors[J].Nat Genet,1997,17 (3):314-317.
  • 4[4]ZUFFEREY R,NAGY D,MANDEL R J,et al.Multiply attenuated lentiviral vector achieved efficient gene delivery in vivo[J].Nat Biotechnol,1997,15 (9):871-875.
  • 5[5]INDRACCOLO S,HABELER W,TISATO V,et al.Gene transfer in ovarian cancer cells:a comparison between retroviral and lentiviral vectors[J].Cancer Res,2002,62(21):6099-6107.
  • 6[6]LI W,XIE S,GUO X,et al.A novel transgenic mouse model produced from lentiviral germline integration for the study of beta-thalassemia gene therapy[J].Haematologica,2008,93(3):356-362.
  • 7[7]LI Z,DULLMANN J,SCHIEDLMEIER B,et al.Murine leukemia induced by retroviral gene marking[J].Science,2002,296:497.
  • 8[8]MODLICH U,KUSTIKOVA O,SCHMIDT M,et al.Leukemia's following retroviral transfer of multidrug resistance 1 are driven by combinatorial insertional mutagenesis[J].Blood,2005,105:4235-4246.
  • 9[9]KUSTIKOVA O S,FEHSE B,MODLICH U,et al.Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking[J].Science,2005,308:1171-1174.
  • 10[10]HACEIN-BEY-ABINA S,VON KALLE C,SCHMIDT M,et al.LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1[J].Science,2003,302:415-419.

同被引文献34

  • 1朱作言,许克圣,谢岳峰,李国华,何玲.转基因鱼模型的建立[J].中国科学（B辑）,1989(2):147-155. 被引量：137
  • 2李艳红,王永华,李燕,杨凌.MDR1基因多态性及其临床相关性研究进展(英文)[J].Acta Genetica Sinica,2006,33(2):93-104. 被引量：11
  • 3张敬之,郭歆冰,谢书阳,朱怡文,黄英,王舒,任兆瑞.用慢病毒载体介导产生绿色荧光蛋白(GFP)转基因小鼠[J].自然科学进展,2006,16(5):571-577. 被引量：25
  • 4LOIS C,HONG E J,PETE S,et al.Germline transmission and tissuespecific expression of transgenes delivered by lentiviral vactors[J].Science,2002,295:868-872.
  • 5PFEIFER A,IKAWA M,DAYN Y,et al.Transgenesis by lentiviral vectors:lack of gene silencing in mammalian embryonic stem cells and preimpalntation embryo[J].Proc Nail Acad Sci USA,2002,99:2140-2145.
  • 6INDRACCOLO S,HABELER W,TISATO V,et al.Gene transfer in ovarian cancer cells:a comparison between retroviral and lentiviral vectors[J].Cancer Res,2002,62(21):6099-6107.
  • 7TRIGLIA T,PETERSONM G,KEMPD J.A Procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences[J].Nucleic Acids Res,1988,16 (16):8186.
  • 8NAKANISHI T,KUROIWA A,YAMADA S,et al.FISH analysis of l42 EGFP transgene integration sites into the mouse genome[J].Genomics,2002,80(6):564-574.
  • 9MISHRA R N,SINGLA-PAREEK S L,NAIR S,et al.Directional genome walking using PCR[J].Biotechniques,2002,33(4):830.
  • 10TAMME R,CAMP E,KORTSCHAK R D,et al.Nonspecific,nested suppression PCR method for isolation of unknown flanking DNA[J].Biotechniques,2000,(5):895-902.

引证文献3

二级引证文献13

医学分子生物学杂志
2008年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部