摘要
目的优化重组人白细胞介素-31(rhIL-31)的表达条件。方法以不同浓度IPTG、不同诱导时间、不同诱导温度对含有pET32a/rhIL-31的工程菌E.coliBL21(DE3)进行诱导表达,用SDS-PAGE对表达产物进行分析。结果诱导时间为5h,IPTG浓度为1.5mmol/L,诱导温度为37℃时,rhIL-31的表达量最高。结论已初步优化了重组蛋白rhIL-31的表达条件。
Objective To optimize the condition for expression of recombinant human interleukin-31 （rhIL-31）. Methods Recombinant E. coli with plasmid pET32a/rhIL-31 was induced at various temperatures with IPTG at different concentrations for different hours, and the expressed protein was identified by SDS-PAGE. Results The expression level of rhIL-31 reached a peak value after the recombinant E. coli was induced with 1. 5 mmol/L IPTG at 37℃ for 5 h. Conclusion The condition for expression of rhIL-31 was preliminarily optimized.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第9期817-819,共3页
Chinese Journal of Biologicals
基金
贵州省社会发展课题项目资助(NY20063039)
关键词
人白细胞介素-31
重组蛋白
表达条件
优化
Human interleukin-31
Recombinant protein
Expression condition
Optimization
