摘要
为了分析1例溶血性输血反应产生的原因,用谱细胞和Le(a-b-)表型细胞鉴定患者血清中的抗体,用抗Lea和抗Leb血型试剂鉴定患者红细胞表型,用PCR测序方法分析LE基因(FUT3基因)全编码区序列。结果表明:患者血清中有抗Lea和抗Leb抗体,血型表型为Le(a-b-),LE基因型为无效等位基因纯合子(le59,508)。结论:抗Leb抗体可引起溶血性输血反应,患者FUT3基因突变导致Le(a-b-)表型。
To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient＇s serum were identified using panel cells and Le（ a - b - ） phenotype cells, patient phenotype was identified by using anti-Le^a and anti- Le^b blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le^a and anti-Le^b antibodies were detected in patient＇s serum. Red cells was typed as Le（ a - b - ） phenotype and the FUT3 genotype was homozygote for non-functional le^59.508 alleles. In conclusion, anti-Le^b antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le^59.508 allele resulting in Le （ a - b - ） phenotype.
出处
《中国实验血液学杂志》
CAS
CSCD
2008年第5期1192-1195,共4页
Journal of Experimental Hematology
基金
浙江省医药卫生科学研究基金,编号2007A044
