摘要
采用免疫组织化学ＡＢＣ法检测了４０例人卵巢浆液性囊腺癌、１０例浆液性囊腺瘤组织中的肿瘤相关基因Ｐ１６、Ｐ５３蛋白表达。结果：Ｐ１６蛋白表达阳性率在肿瘤分化Ⅰ、Ⅱ、Ⅲ级中，分别为１６．６７％、３３．３３％和５２．６３％。４０例浆液性囊腺癌Ｐ１６蛋白表达均为阴性。Ｐ１６蛋白阳性表达与肿瘤组织学分级无明显关系（Ｐ＞０．０５）。４０例同组病例中，Ｐ５３蛋白总阳性表达率为６０％，１０例浆液性囊腺瘤Ｐ５３蛋白均呈阴性表达。低分化浆液性囊腺癌的Ｐ５３阳性表达率（７５％），高于肿瘤分化高者（３５．７１％）。两者差异有显著性（Ｐ＜０．０５）。Ⅰ与Ⅱ、Ⅱ与Ⅲ级肿瘤间。
An immunohistochemical method utilizing avidinbiontin complex (ABC) technique was used in this study to detect P16 and P53 protein expressions in 40 ovarian serous cystadenocarcinomas and 10 serous cystadenomas.The results showed that the total positive rates of P16 and P53 protein were 40% and 60% respectively.The positive staining rate was higher in P53 protein expression than in P16 protein expression in the same cases.The positive staining rates of P16 protein were 16.67%,33.33% and 52.63% respectively in stages Ⅰ,Ⅱ and Ⅲ.There was no significant difference in histological staging.The postive staining rate of P53 protein expression in poorly differentiated tumor was higher than that in well differentiated group (P<005)These results suggested that P16 and P53 protein expressions of the ovarian serous cystadenocarcinoma might be correlated with human organs,histological type of tumor,cyclin and cyclindependent kinase.
出处
《华西医科大学学报》
CSCD
1997年第4期417-419,433,共4页
Journal of West China University of Medical Sciences
