摘要
目的：用磁性微粒分离免疫酶联法测定血清（浆）促卵泡激素（ＦＳＨ）和促黄体生成素（ＬＨ），以对该方法的方法学性能作一评价。方法：以临床患者空腹血作为检测标本，用磁性微粒酶联免疫法检测血中的ＦＳＨ和ＬＨ，观察其线性范围、试剂显色稳定性、精密度、准确度以及与放免配对比较等。结果：以ＦＳＨ为例，其线性范围０～１５０ＩＵ／Ｌ；显色稳定性：空白管ＣＶ为２．３％，测定管ＣＶ为０．４％；批内变异２．２９％，批间变异８．８５％；回收率为９３．５％；与ＲＩＡ比较相关系数为０．９８２５，Ｐ＜０．００１。结论：该方法具有重复性好，特异性高，准确可靠，与ＲＩＡ具有良好的相关性，且无放射性污染，值得推荐。
Objective:To detect serum follicle stimulating hormone(FSH) and luteinizing hormone(LH) with magnetic antibody enzyme linking immunoassay(MAIA).Methods:Serum FSH and LH were detected with MAIA according to the direction of instrument and commercial kits.Their linearity,stability in color formation,precision and accuracy were evaluated and compared with RIA.Results:The range of linearity,for example,in FSH,was 0～150 IU/L;stability of color:blank tube CV 2.3%,unknown tube CV 0.4%;intra assay( n =10) and inter assay( n =5) CV 2.29% and 8.85% respectively;the rate of recovery was 93.5%;the relative coefficient( r ) 0.982 5 (compared with RIA), P <0.001.Conclusions:These results indicate that this method has many advantages over RIA and is worthy of recommendation.
出处
《蚌埠医学院学报》
CAS
1997年第6期425-426,共2页
Journal of Bengbu Medical College
关键词
血清
促卵泡激素
促黄体生成素
ELISA
serum
follicle stimulating hormone(FSH)
luteinizing hormone(LH)
magnetic antibody immunoassay(MAIA)
