摘要
背景:前期研究已经成功制备了硫酸钙/冻干骨复合型生物多孔支架,但其表面的疏水性不利于细胞黏附、增殖。目的:观察Ⅰ型胶原表面修饰对硫酸钙/冻干骨复合型生物多孔支架亲水性和细胞相容性的影响。设计、时间及地点:体外细胞学对比观察,于2007-10/2008-06在山西医科大学第二医院骨科实验室及山西医科大学中心实验室完成。材料:应用Ⅰ型胶原进行表面修饰制备复合Ⅰ型胶原的硫酸钙/冻干骨复合型生物多孔支架。方法:将骨髓间充质干细胞分别与原支架(对照组)和表面修饰后的支架(实验组)复合培养。主要观察指标:测定表面修饰后支架的亲水性;扫描电镜观察表面改性后的材料及细胞与材料复合的形态学特征;分别使用MTT法、细胞蛋白质及碱性磷酸酶定量方法对细胞与材料复合培养后增殖与分化能力进行评估。结果:表面修饰后的新型多孔硫酸钙支架的亲水率高于原支架(P<0.05)。实验组细胞的黏附率高于对照组(P<0.05);在培养的不同时期实验组的细胞数量、细胞的蛋白质含量及细胞碱性磷酸酶表达量均较对照组多(P<0.05)。结论:Ⅰ型胶原表面修饰后的硫酸钙/冻干骨复合型生物多孔支架的亲水性和细胞相容性得到了显著提高。
BACKGROUND： Previous researches have successfully prepared a bioactive porous scaffold of calcium sulfate/freeze-dried bone, but its surface hydrophobicity limits the cell proliferation. OBJECTIVE： To investigate the influence on the hydrophobicity and compatibility of bioactive porous scaffold composed of calcium sulfate and freeze-dried bone, which is modified with collagen type Ⅰ . DESIGN, TIME AND SETTING： A control trial in vitro was carried out in the Orthopaedic Laboratory and Central Laboratory of Second Hospital Affiliated to Shanxi Medical University from October 2007 to June 2008. MATERIALS： Bioactive porous scaffold composed of calcium sulfate and freeze-dried bone was prepared by the surface modification with collagen type Ⅰ. METHODS： Bone marrow mesenchymal stem cells were co-cultured with primary scaffold （control group） and modified scaffold （experiment group）. MAIN OUTCOME MEASURES： The hydrophobicity of the modified scaffold was determined; The morphology of cells on the surface of bioactive porous scaffold with collagen type Ⅰ was studied by scanning electron microscopy （SEM）; Cell viability was assessed by MTT and measuring the cell protein; The differentiated osteoblastic function was assessed by measuring alkaline phosphatase activity of the cells. RESULTS： The collagen type Ⅰ composite coating could improve cell proliferation, protein content and alkaline phosphatase activity of the hybrid scaffolds and increase its hydrophilicity after surface modification （P 〈 0.05）. CONCLUSION： The hydrophobicity and compatibility of bioactive porous scaffold composed of calcium sulfate and freeze-dried bone, which is modified with collagen type Ⅰ , have been improved.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第36期7127-7130,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
